Low levels of antibodies against E-coli and mycobacterial 65kDa heat shock proteins in patients with inflammatory bowel disease
Low levels of antibodies against E-coli and mycobacterial 65kDa heat shock proteins in patients with inflammatory bowel disease
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DOI：
10.1007/s00011-004-1296-7
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发表时间：
2004-10-01
影响因子：
6.7
通讯作者：
Prohászka, Z
中科院分区：
文献类型：
--
作者：
Huszti, Z;Bene, L;Prohászka, Z
目的和设计：本研究的目的是支持和扩展我们最初的观察，我们在炎症性肠病（IBD）患者中发现低水平的抗分枝杆菌65kD热休克蛋白抗体。为此，我们对124名IBD患者进行了新的测试，除了测量先前描述的牛分枝杆菌65kD热休克蛋白（Hsp65）和人60kD热休克蛋白（Hsp60）的抗体外，我们还测定了大肠杆菌Hsp65的IgG抗体水平，称为GroEL。患者和对照组：74例克罗恩病（CD）患者（男性30例，女性44例，年龄33（27-45）岁，中位数（四分位数间距））和50例溃疡性结肠炎（UC）患者（男性22例，女性28例，年龄38（30-50）岁）纳入研究。110名健康受试者作为对照，其中男性34人，女性76人，47人（37 ~ 53岁）。研究对象是在1131中心接受复杂治疗的连续患者。方法与统计分析：采用ELISA法检测igg型抗体与伴侣蛋白60家族蛋白反应的数量。由于热休克蛋白抗体水平作为变量不是正态分布，因此采用非参数Mann-Whitney检验和Dunn事后检验进行组间比较。结果：IBD患者抗GroEL（7,5(3,5-18,3)）和抗Hsp65（4,8(2,1-7,85)）的中位水平显著低于健康者(GroEL: 10,0 (5,4-3 1,0)；Hsp65: 7,04(4,66-12,77))。然而，这种差异被发现仅限于CD患者(GroEL: 7,5 (3,7-14,2)；P < 0.05；Hsp65: 4,35 (1,90-6,94)；P < 0.001)。我们未发现患者（Hsp60: 45,5(24,9-69,0)）和健康对照组（38,4(21,6-69,4)）之间的抗人Hsp60 IgG水平有差异。在疾病的活动性和非活动性阶段，各抗体的血清浓度无显著差异。结论：我们的研究结果支持了我们之前工作的结论，牛分枝杆菌hsp65抗体水平下降，大肠杆菌GroEl抗体水平也下降。相反，人抗hsp60抗体的浓度没有变化。这些发现表明，65 kDa细菌热休克蛋白抗体的产生在IBD中选择性受损。
Objective and design: The aim of the present study was to support and extend our initial observation, where we found low levels of antibodies against mycobacterial 65kD heat shock proteins in patients with inflammatory bowel disease (IBD). For this purpose we tested a new group of 124 patients with IBD, and beside measuring antibodies to Mycobacterium bovis 65kD heat shock protein (Hsp65) and human 60kD, heat shock protein (Hsp60) as described previously, we also determined IgG antibody levels to Hsp65 from E. coli, called GroEL.Patients and control subjects: seventy-four patients with Crohn's disease (CD) (30 males, 44 females, 33 (27-45) years old, median (interquartile range)) and 50 patients with ulcerative colitis (UC) (22 males, 28 females, 38 (30-50) years old) were involved in the study. 110 healthy subjects (34 males, 76 females, 47 (37-53) years old) served as controls. Study subjects were consecutive patients referred to an 1131) center for complex treatment of the disease.Methods and statistical analysis: The amounts of IgG-type antibodies reacting with proteins of the chaperonin 60 family were assessed by ELISA. Since the antibody levels to heat-shock proteins as variables were not normally distributed, non-parametric Mann-Whitney test and Dunn post hoc test were used for group comparisons.Results: Median levels of anti-GroEL (7,5 (3,5-18,3)) and anti-Hsp65 (4,8 (2,1-7,85)) were significantly (GroEL p = 0,008; and Hsp65 p < 0,001) lower in the IBD patients than in the healthy subjects (GroEL: 10,0 (5,4-3 1,0); Hsp65: 7,04 (4,66-12,77)). However this difference was found to be restricted to the CD patients (GroEL: 7,5 (3,7-14,2); p < 0,05; Hsp65: 4,35 (1,90-6,94); p < 0,001). We did not find difference in the concentration of anti-human Hsp60 IgG levels between patients (Hsp60: 45,5 (24,9-69,0)) and healthy controls (38,4 (21,6-69,4). Regarding the serum concentrations of each antibody tested there was no significant difference between the active and inactive stage of disease.Conclusion: Our present findings support conclusion of our previous work, antibody levels not only for Mycobacterium bovis hsp65 but for E. coli GroEl were found to be decreased as well. In contrast no changes in the concentrations of human anti-hsp60 antibodies were observed. These findings indicate that production of antibodies to 65 kDa bacterial heat shock proteins is selectively impaired in IBD.