Cyanobacteria-specific algicidal mechanism of bioinspired naphthoquinone derivative, NQ 2-0.
Cyanobacteria-specific algicidal mechanism of bioinspired naphthoquinone derivative, NQ 2-0.
复制标题
DOI：
10.1038/s41598-018-29976-5
复制
发表时间：
2018-08-02
影响因子：
4.6
通讯作者：
Han MS
中科院分区：
文献类型：
--
作者：
Lee HW;Park BS;Joo JH;Patidar SK;Choi HJ;Jin E;Han MS
为了抑制蓝藻水华，已经开发了对蓝藻具有选择性杀藻活性的萘醌衍生物NQ 2-0。然而，由于缺乏有关其杀藻机制的信息，我们对这种物质如何能够选择性杀死蓝藻的理解存在重大差距。在这里，我们研究了NQ 2-0的选择性杀藻机制，使用目标（铜绿微囊藻）和非目标（小环藻和羊角月牙藻）物种。NQ 2-0对M.铜绿假单胞菌，这种活性是强烈的光依赖性。该NQ化合物选择性地降低了M的氧释放速率和光系统II（PSII）效率。铜绿假单胞菌通过阻断来自光合电子传递系统的电子传递，并显著（p ≤ 0.05）增加活性氧（ROS）水平，导致膜通过脂质过氧化损伤。超微结构观察表明，NQ 2-0处理后12 h内类囊体膜解体，24 h细胞质空泡化，细胞膜解体。这些发现表明，NQ 2-0处理后增加的ROS水平可诱导细胞死亡。有趣的是，与非靶真核细胞相比，M.铜绿假单胞菌表现出相对较晚的抗氧化反应，以减少增加的ROS水平，这可能会增强对这种蓝藻的杀藻活性。
To mitigate cyanobacterial blooms, the naphthoquinone derivative, NQ 2-0, which has selective algicidal activity against cyanobacteria, has been developed. However, due to a lack of information on its algicidal mechanisms, there are significant gaps in our understanding of how this substance is capable of selectively killing cyanobacteria. Here, we investigated the selective algicidal mechanisms of NQ 2-0 using target (Microcystis aeruginosa) and non-target (Cyclotella sp. and Selenastrum capricornutum) species. NQ 2-0 showed selective algicidal activity against only M. aeruginosa, and this activity was strongly light-dependent. This NQ compound has selectively reduced the oxygen evolution rate and photosystem II (PSII) efficiency of M. aeruginosa throughout blocking electron transfer from the photosynthetic electron transport system, and significantly (p ≤ 0.05) increased levels of reactive oxygen species (ROS), resulting in membrane damage through lipid peroxidation. In ultrastructural observations, thylakoid membranes were disintegrated within 12 h after NQ 2-0 treatment, and cytoplasmic vacuolation and disintegrated cellular membrane were observed at 24 h. These findings suggest that increased ROS levels following NQ 2-0 treatment may induce cell death. Interestingly, compared to non-target eukaryotic cells, M. aeruginosa showed relatively late antioxidant response to reduce the increased ROS level, this may enhance algicidal activity against this cyanobacterium.
登录
查看更多内容
影响因子：
3
作者：
Kim, Yeon-Mi;Wu, Ying;Jin, EonSeon
通讯作者：
Jin, EonSeon
DOI：
10.1111/j.1438-8677.2008.00078.x
发表时间：
2008-11
期刊：
Plant biology (Stuttgart, Germany)
影响因子：
--
作者：
Darehshouri A;Affenzeller M;Lütz-Meindl U
通讯作者：
Lütz-Meindl U
影响因子：
2.6
作者：
Hill, Geoffrey E.
通讯作者：
Hill, Geoffrey E.
影响因子：
6.8
作者：
Joo, Jae-Hyoung;Wang, Pengbin;Han, Myung-Soo
通讯作者：
Han, Myung-Soo
影响因子：
4.5
作者：
Perschbacher, PW;Ludwig, GM
通讯作者：
Ludwig, GM