PHOTODYNAMICALLY INDUCED CYTOTOXICITY OF HYPERICIN DYE ON HUMAN FIBROBLAST CELL-LINE MRC5
PHOTODYNAMICALLY INDUCED CYTOTOXICITY OF HYPERICIN DYE ON HUMAN FIBROBLAST CELL-LINE MRC5
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DOI：
10.1016/1011-1344(94)07064-u
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发表时间：
1995-02-01
影响因子：
5.4
通讯作者：
JARDON, P
中科院分区：
文献类型：
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作者：
HADJUR, C;RICHARD, MJ;JARDON, P
使用人成纤维细胞系 MRC5 研究了金丝桃素 (hyp) 在癌症光动力疗法 (PDT) 中的可能应用。在有氧条件下，pH 7.4，用不同剂量的可见光和不同剂量的hyp照射MRCS细胞。低浓度的hyp (5x10(-9) M)对MRC5细胞有很强的毒性，在40分钟的照射时间内产生15%的存活率。在黑暗中，在10(-9)-10(-7) M hyp范围内没有观察到细胞毒性。还检查了 hyp 杀死细胞的机制。添加 1,4-二氮杂双环[2,2,2]辛烷 (DABCO) 或组氨酸（已知的 II 型机制猝灭剂）可显着抑制 MRCS 杀伤。此外，氧化氘增强了hyp的光动力效应。添加去铁胺、过氧化氢酶或超氧化物歧化酶(SOD)，已知的I型机制的清除剂，对hyp的光动力作用速率具有显着的抑制作用。实验结果表明，hyp 作为癌症 PDT 的敏化剂具有相当大的潜力。
The possible application of hypericin (hyp) in the photodynamic therapy (PDT) of cancer was investigated using the human fibroblast cell line MRC5. In aerobic conditions, at pH 7.4, irradiation of MRCS cells was carried out with different doses of visible light and different doses of hyp. A low concentration of hyp (5x10(-9) M) was highly toxic to MRC5 cells, producing 15% survival for an irradiation period of 40 min. In the dark, no cytotoxicity was observed in the range 10(-9)-10(-7) M hyp. The mechanism of cell killing by hyp was also examined. Significant inhibition of MRCS killing was observed on addition of 1,4-diazabicyclo[2,2,2]octane (DABCO) or histidine, known quenchers of type II mechanisms. In addition, the photodynamic effect of hyp was enhanced by deuterium oxide. The addition of desferrioxamine, catalase or superoxide dismutase (SOD), known scavenging agents of the type I mechanism, had a significant inhibitory effect on the rate of photodynamic action of hyp. The experimental results suggest that hyp has considerable potential for use as a sensitizer in the PDT of cancer.