MITOCHONDRIAL-DNA SEQUENCES IN THE NUCLEAR GENOME OF STRONGYLOCENTROTUS-PURPURATUS
MITOCHONDRIAL-DNA SEQUENCES IN THE NUCLEAR GENOME OF STRONGYLOCENTROTUS-PURPURATUS
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DOI：
10.1016/s0022-2836(83)80270-8
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发表时间：
1983-01-01
影响因子：
5.6
通讯作者：
DAVIDSON, EH
中科院分区：
文献类型：
--
作者：
JACOBS, HT;POSAKONY, JW;DAVIDSON, EH
两个海胆胚胎互补[c]DNA克隆线粒体16 S rRNA和细胞色素氧化酶亚基I mRNA的特点。克隆的cDNA与海胆线粒体DNA共线，它们的鉴定是基于与已知的人线粒体DNA限制性片段的交叉杂交，以及核苷酸序列测定。线粒体cDNA克隆在凝胶印迹杂交中也显示出与特定基因组DNA序列的意外反应。基因组噬菌体分离含有与线粒体克隆杂交的序列的重组体，并确定这些序列的排列。所研究的基因组区域包含与线粒体16 S rRNA基因的3“末端同源的序列，一侧可能是细胞色素氧化酶亚基I基因的完整拷贝，另一侧是该基因片段的重复。细胞色素氧化酶亚基I基因的线粒体和核同源物之间的核苷酸序列差异在基因的不同区域变化，约为13- 25%，而3“16 S rRNA序列的核和线粒体版本之间存在约8%的序列差异。基因组线粒体序列同源物的结构表明，在海胆进化过程中，发生了一个生殖系转座的线粒体基因组片段到核DNA，然后重排和单核苷酸取代。
Two sea urchin embryo complementary [c]DNA clones representing mitochondrial 16 S rRNA and cytochrome oxidase subunit I mRNA were characterized. The cloned cDNA are colinear with sea urchin mitochondrial DNA, and their identification is based on cross-hybridization with known restriction fragments of human mitochondrial DNA, and on nucleotide sequence determinations. The mitochondrial cDNA clones also displayed an unexpected reaction with specific genomic DNA sequences in gel blot hybridizations. Genomic phage .lambda. recombinants containing sequences hybridizing with the mitochondrial clones were isolated and the arrangement of these sequences was determined. The genomic region studied contains a sequence homologous with the 3'' end of the mitochondrial 16 S rRNA gene, flanked on one side by what is possibly a complete copy of the cytochrome oxidase subunit I gene, and on the other by a duplication of a fragment of this gene. The nucleotide sequence divergence between the mitochondrial and nuclear homologues of the cytochrome oxidase subunit I gene varies for different regions of the gene, from about 13-25%, while there is about 8% sequence divergence between nuclear and mitochondrial versions of the 3'' 16 S rRNA sequence. The structure of the genomic mitochondrial sequence homologues indicates that during sea urchin evolution there occurred a germ-line transposition of a fragment of the mitochondrial genome into the nuclear DNA, followed by rearrangements and single nucleotide substitutions.