Synthesis of activated 3′-amino-3′-deoxy-2-thio-thymidine, a superior substrate for the nonenzymatic copying of nucleic acid templates
Synthesis of activated 3′-amino-3′-deoxy-2-thio-thymidine, a superior substrate for the nonenzymatic copying of nucleic acid templates
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DOI：
10.1039/c5cc10317g
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发表时间：
2016-01-01
影响因子：
4.9
通讯作者：
Szostak, Jack W.
中科院分区：
文献类型：
--
作者：
Izgu, Enver Cagri;Oh, Seung Soo;Szostak, Jack W.
我们提出了一种可扩展的合成3 '-氨基-3'-脱氧-2-硫代-胸苷-5 '-磷酸-2-甲基咪唑，一种活化的单体，可以在没有聚合酶的情况下快速复制核酸模板中的腺苷残基。与3 '-氨基-3'-脱氧-T单体相比，硫原子取代使模板复制速率提高5倍，而与它们的核糖核苷酸对应物相比，3 '-氨基单体表现出2至3'-倍的提高。
We present a scalable synthesis of 3'-amino-3'-deoxy-2-thio-thymidine-5'-phosphoro-2-methylimidazolide, an activated monomer that can copy adenosine residues in nucleic acid templates rapidly without a polymerase. The sulfur atom substitution enhances the rate of template copying by 5-fold compared with the 3'-amino-3'-deoxy-T monomer, while the 3'-amino monomers exhibit a 2- to 3'-fold enhancement compared with their ribonucleotide counterparts.