Identification and characterization of a virus-inducible non-coding RNA in mouse brain
Identification and characterization of a virus-inducible non-coding RNA in mouse brain
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DOI：
10.1099/vir.0.81768-0
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发表时间：
2006-07-01
影响因子：
3.8
通讯作者：
Rangarajan, Pundi N.
中科院分区：
文献类型：
--
作者：
Saha, Sougata;Murthy, Sreenivasa;Rangarajan, Pundi N.
用日本脑炎病毒或狂犬病病毒感染小鼠导致小鼠中枢神经系统中编码新的非编码RNA（ncRNA）的基因的激活。该转录物，命名为病毒诱导型ncRNA（VINC），与在小鼠新生儿皮肤中表达的3.18 kb转录物（GenBank登录号AK028745）相同，其与许多未注释的cDNA和表达的序列标签一起被分组在小鼠单基因簇Mm281895中。VINC在早期小鼠胚胎和几种成年非神经元小鼠组织以及鼠肾腺癌（RAG）细胞系中组成型表达。细胞核和细胞质RNA的北方印迹显示，VINC主要定位于RAG细胞的细胞核中，因此是核ncRNA家族的新成员。
Infection of mice with Japanese encephalitis virus or Rabies virus results in the activation of a gene encoding a novel, non-coding RNA (ncRNA) in the mouse central nervous system. This transcript, named virus-inducible ncRNA (VINC), is identical to a 3.18 kb transcript expressed in mouse neonate skin (GenBank accession no. AK028745) that, together with a number of unannotated cDNAs and expressed sequence tags, is grouped in the mouse unigene cluster Mm281895. VINC is expressed constitutively in early mouse embryo and several adult non-neuronal mouse tissues, as well as a murine renal adenocarcinoma (RAG) cell line. Northern blotting of nuclear and cytoplasmic RNAs revealed that VINC is localized primarily in the nucleus of RAG cells and is thus a novel member of the nuclear ncRNA family.