miR-7 inhibits colorectal cancer cell proliferation and induces apoptosis by targeting XRCC2.
miR-7 inhibits colorectal cancer cell proliferation and induces apoptosis by targeting XRCC2.
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miR-7 通过靶向 XRCC2 抑制结直肠癌细胞增殖并诱导细胞凋亡。
DOI：
10.2147/ott.s59364
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发表时间：
2014
影响因子：
4
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Xu K;Chen Z;Qin C;Song X
使用公开可用的算法进行的分析预测，中国仓鼠细胞2（XRCC 2）中的X射线修复补充缺陷修复（同源重组修复途径中的关键组分）是微核糖核酸-7（miR-7）的潜在靶点。一些研究表明，miR-7和XRCC 2都与癌症的发生有关。为此，我们寻找miR-7和XRCC 2在结直肠癌（CRC）发展中的可能关系。使用实时聚合酶链反应（PCR）评估CRC标本和细胞系中的miR-7表达。荧光素酶报告基因测定用于确认靶缔合。通过MTT法、集落形成法和流式细胞术在体外证实miR-7对细胞增殖和凋亡的影响。分别用真实的时间PCR和蛋白质印迹法检测基因和蛋白质表达。miR-7在CRC标本和细胞系中下调，靶向XRCC 2的3′非翻译区。miR-7过表达降低cyclin D1表达，增加p21、caspase-3和BAX表达，随后抑制CRC细胞增殖并诱导CRC细胞凋亡。然而，XRCC 2可以抑制miR-7对增殖的抑制作用。我们的研究结果表明，miR-7通过抑制CRC细胞的增殖和增加其凋亡而发挥保护作用。它可以确定抗癌治疗的新靶点。
Analysis using publicly available algorithms predicts that X-ray repair complementing defective repair in Chinese hamster cells 2 (XRCC2), a key component in the homologous recombination repair pathway, is a potential target of micro-ribonucleic acid-7 (miR-7). Some studies have shown that both miR-7 and XRCC2 are associated with cancer development. For this purpose, we searched for the possible relationship between miR-7 and XRCC2 in the development of colorectal cancer (CRC). miR-7 expression was assessed in CRC specimens and cell lines using real-time polymerase chain reaction (PCR). Luciferase reporter assay was used to confirm the target associations. The effect of miR-7 on cell proliferation and apoptosis was confirmed in vitro by the methylthiazol tetrazolium (MTT) assay, colony formation assay, and flow cytometry. Gene and protein expression were examined using real time PCR and western blotting, respectively. miR-7 was downregulated in CRC specimens and cell lines, and targeted the 3′ untranslated region of XRCC2. miR-7 overexpression reduced cyclin D1 expression and increased p21, caspase-3, and BAX expression, which subsequently inhibited CRC cell proliferation and induced CRC cell apoptosis. However, XRCC2 can repress the inhibitory effects of miR-7 on proliferation. Our findings suggest that miR-7 plays a protective role by inhibiting proliferation and increasing apoptosis of CRC cells. It may identify new targets for anti-cancer treatment.