Gp85 genetic diversity of avian leukosis virus subgroup J among different individual chickens from a native flock
Gp85 genetic diversity of avian leukosis virus subgroup J among different individual chickens from a native flock
复制标题
本地鸡群不同鸡个体间禽白血病病毒J亚型的gp85遗传多样性
DOI：
10.3382/ps/pew407
复制
发表时间：
2017-05-01
期刊：
影响因子：
4.4
通讯作者：
Zhao，Peng
中科院分区：
文献类型：
--
作者：
Li，Yang;Fu，Jiayuan;Zhao，Peng
&NA;为比较不同个体间禽白血病病毒（Avian leukosis virus，ALV）的遗传多样性和准种进化，从山东省某鸡群中随机抽取5只ALV进行了研究。采集血样，接种鸡胚成纤维细胞和DF-1细胞系，分别进行马立克氏病病毒（MDV）、网状内皮组织增生病病毒（REV）和ALV的病毒分离和鉴定。对5株J亚群禽白血病病毒（ALV-J）的gp 85基因进行了扩增和克隆。对每株菌分别选取20个gp 85基因阳性克隆进行序列分析，比较5株菌准种的变异性。结果表明，5株ALV-J分离株gp 85基因的核苷酸长度分别为921 bp、921 bp、924 bp、918 bp和912 bp，氨基酸同源性分别为99.3 ~ 100%、99.3 ~ 100%、99.4 ~ 100%、98.4 ~ 100%、99.3 ~ 100%、99.4 ~ 100%、99.4 ~ 100%、99.3 ~ 100%、99.3 ~ 100%、99.4 ~ 100%、99.3 ~ 100%、99.4 ~ 100%、99.4 ~ 100% 99.0 - 100%。优势准种的比例分别为65.0%、85.0%、85.0%、50.0%、84.2%，这些优势准种之间gp 85基因的同源性为89.2 ~ 92.5%。这些数据表明，即使在同一群内，ALV-J准种的组成在感染个体之间也各不相同，并且优势准种的比例和基因突变都在继续进化。
&NA; To compare the genetic diversity and quasispecies evolution of avian leukosis virus (ALV) among different individuals, 5 chickens, raised in Shandong Provice of China, were randomly selected from a local chicken flock associated with serious tumor cases. Blood samples were collected and inoculated into chicken embryo fibroblast and DF‐1 cell lines for virus isolation and identification, respectively, of Marek's disease virus (MDV), reticuloendotheliosis virus (REV), and ALV. Five strains of ALV subgroup J (ALV‐J) were identified, and the gp85 gene from each strain was amplified and cloned. For each strain, about 20 positive clones of gp85 gene were selected for sequence analyses and the variability of the quasispecies of the 5 strains was compared. The results showed that the nuclear acid length of gp85 gene of 5 ALV‐J isolates is 921 bp, 921 bp, 924 bp, 918 bp, and 912 bp respectively, and amino acid homologies of different gp85 clones from the 5 ALV‐J strains were 99.3 to 100%, 99.3 to 100%, 99.4 to 100%, 98.4 to 100%, 99.0 to 100%, respectively. The proportions of dominant quasispecies were 65.0%, 85.0%, 85.0%, 50.0%, 84.2%, respectively, and homology of the gp85 among these dominant quasispecies was 89.2 to 92.5%. These data demonstrated the composition of the ALV‐J quasispecies varied among infected individuals even within the same flock, and the dominant quasispecies continued to evolve both for their proportion and gene mutation.