PURIFICATION OF 5 MAIN CALF THYMUS HISTONE FRACTIONS BY GEL EXCLUSION CHROMATOGRAPHY
PURIFICATION OF 5 MAIN CALF THYMUS HISTONE FRACTIONS BY GEL EXCLUSION CHROMATOGRAPHY
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DOI：
10.1016/0014-5793(73)80797-5
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发表时间：
1973-01-01
期刊：
影响因子：
3.5
通讯作者：
VONHOLT, C
中科院分区：
文献类型：
--
作者：
BOHM, EL;STRICKLAND, WN;VONHOLT, C
离子ex - change~ s (CMC-eeltu) ~ ose和Aznbez！tRC-50)和~ _分子。~ sSev~ s （Sephadex和~ l B~ gel）已被广泛应用于历史色谱（参考文献见1]）- b0 ~ me~ ods已被成功地用于纯化组蛋白f~ ac ~离子和n~ jx0s。然而，目前的x射线分形方法还不能将其全部分解成5个主要部分。最近的借口越来越多。在Biogel P-60上进行层析，得到了高纯度的Fa_和F2a，留下了F3、fz2和F2b未解析的[3]。在生物凝胶p -10# yes F]上进行层析，经过适当的po~ ling和zehchromatography后，p~ a~ e ~ he ~ ones~ 4]。
Ion ex. change~ s (CMC-eeltu~ ose and Aznbez! ite tRC-50) and~ _nolecula.~ sSev~ s (Sephadex an~ l B~ egel) have been widely used for~ he chromatography of histories (for references see I]])-B0~ me~ ods have been successfully t'mp,] oye, d for* he purification of histone f~ ac-~ ions and n~ Jxtures obtained by the nae hod of Johns [2]. However~ at presen~ ch, xomatogmphic methods~ 1one can not iesolve whole his lone into i~ s five main fractions [1]. Recently excaus~ on ehr. omatography on Biogel P-60 has yielded Fa_ and F2a 1 ha high purity, leaving F3, Fza 2 a~ d F2b unresolved [3]. Chromatography on Biogel P-10# yes F] and, after appropriate po~ ling and zeehrornatography, o, lhei p~ a~ e his~ ones~ 4].