RhBMP-2 immobilized on poly(phthalazinone ether nitrile ketone) via chemical and physical modification for promoting in vitro osteogenic differentiation
RhBMP-2 immobilized on poly(phthalazinone ether nitrile ketone) via chemical and physical modification for promoting in vitro osteogenic differentiation
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DOI：
10.1016/j.colsurfb.2020.111173
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发表时间：
2020-10-01
影响因子：
5.8
通讯作者：
Jian, Xigao
中科院分区：
文献类型：
--
作者：
Liu, Wentao;Wang, Han;Jian, Xigao
聚醚醚酮（PEEK）是一种常用于骨植入物的聚芳醚酮，但PEEK表面改性困难。相反，聚（二氮杂萘酮醚腈酮）（PPENK）是聚芳醚酮，其表面由于其氰基而可以通过使用化学反应进行改性。本文通过共价固定和肝素结合rhBMP-2的方法制备了两种材料P-BMP-2和PH-BMP-2，以增强PPENK的成骨活性。用X射线光电子能谱和水接触角测量法研究了PPENK上氰基的水解、胺基的接枝和rhBMP-2的固定化。免疫组织化学染色和评价的负载和释放行为被用来证明PPENK表面上的rhBMP-2的存在。采用细胞粘附、存活和增殖试验评价MC 3 T3-E1前成骨细胞在样品上的生物学活性。通过逆转录聚合酶链反应评估与成骨活性相关的基因的基因表达。体外实验结果表明，P-BMP-2和PH-BMP-2均具有良好的细胞相容性，并能促进MC 3 T3-E1前成骨细胞的分化。特别地，使用肝素结合方法可以获得有利的生物相容性。
Poly(ether ether ketone) (PEEK) is a polyaryletherketone commonly used for bone implants, but it is difficult to modify the PEEK surface. Conversely, poly(phthalazinone ether nitrile ketone) (PPENK) is a polyaryletherketone whose surface can be modified by using chemical reactions owing to its cyano group. In this paper, two types of materials, P-BMP-2 and PH-BMP-2, were prepared by covalent immobilization and heparin binding of rhBMP-2 respectively to enhance the osteogenic activity of PPENK. X-ray photoelectron spectroscopy and water contactangle measurement were used to demonstrate the hydrolysis of the cyano groups on PPENK, amine group grafting and immobilization of rhBMP-2. Immunohistochemical staining and evaluation of loading and release behaviour were used to demonstrate the existence of rhBMP-2 on PPENK surfaces. The biological activity of MC3T3-E1 preosteoblast cells on the samples were evaluated using cell adhesion, viability and proliferation tests. The genetic expression of genes associated with osteogenic activity was assessed by reverse transcription polymerase chain reaction. Based on the obtained in vitro experimental results, both P-BMP-2 and PH-BMP-2 exhibit good cytocompatibility and promote differentiation of MC3T3-E1 preosteoblast cells. In particular, the favourable biocompatibility can be obtained using the heparin-binding method.