CHEMICAL COMPOSITION OF CYTOPLASMIC MEMBRANE OF BACILLUS SUBTILIS

CHEMICAL COMPOSITION OF CYTOPLASMIC MEMBRANE OF BACILLUS SUBTILIS
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DOI:
10.1111/j.1432-1033.1967.tb00158.x
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发表时间:
1967-01-01
期刊:
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子:
--
通讯作者:
SAMUELSSON, B
SAMUELSSON, B
中科院分区:
其他
文献类型:
--
作者:
BISHOP, DG;RUTBERG, L;SAMUELSSON, B

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B的细胞质膜。枯草芽孢杆菌168,从细胞在稳定期制备,含有蛋白质(62%),RNA(22%)和脂质(16%)。膜脂质分析显示存在磷脂(75%)、中性脂质(10%)和一种鉴定为二葡糖基甘油二酯的化合物。主要的磷脂是二磷脂酰甘油和磷脂酰乙醇胺,以及少量的磷脂酰甘油和脂肪氨基酸。支链脂肪酸占全细胞和细胞膜总脂肪酸的75%以上。主要成分为15和17个碳原子的异酸和反异酸,以及少量的14和16个碳原子的异酸和正酸。不存在不饱和酸。
The cytoplasmic membrane of B. subtilis 168, prepared from cells in the stationary phase, contained protein (62%), RNA (22%) and lipid (16%). Analyses of the membrane lipid revealed the presence of phospholipids (75%), neutral lipid (10%) and a compound identified as a diglucosyl diglyceride. The major phospholipids were diphosphatidyl glycerol and phosphatidyl ethanol-amine, with small amounts of phosphatidyl glycerol and lipoamino acids. Branched chain fatty-acids comprised over 75% of the total fatty acids of both whole cells and membranes. Iso and anteiso acids with 15 and 17 carbon atoms were the major components, together with small amounts of iso acids containing 14 and 16 carbon atoms and n-acids. No unsaturated acids were present.