LIMITING DILUTION ANALYSIS OF THE FREQUENCY OF ANTIGEN-REACTIVE LYMPHOCYTES ISOLATED FROM THE CENTRAL-NERVOUS-SYSTEM OF LEWIS RATS WITH EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS
LIMITING DILUTION ANALYSIS OF THE FREQUENCY OF ANTIGEN-REACTIVE LYMPHOCYTES ISOLATED FROM THE CENTRAL-NERVOUS-SYSTEM OF LEWIS RATS WITH EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS
复制标题
DOI：
10.1016/0008-8749(87)90204-8
复制
发表时间：
1987-08-01
影响因子：
4.3
通讯作者：
LISAK, RP
中科院分区：
文献类型：
--
作者：
COHEN, JA;ESSAYAN, DM;LISAK, RP
在用髓鞘碱性蛋白（MBP）和破伤风类毒素（TT）免疫12天后，从患有急性实验性过敏性脑脊髓炎（EAE）的Lewis大鼠的脊髓和引流淋巴结中分离出淋巴细胞。平均分 8.0 .+-。 2.0.次。从脊髓中获得106个细胞。其中 71.1 .+-. 8.6% 表达辅助 T 细胞标记 W3/25 和 14.8.+-。 6.2% 表达杀伤/抑制 T 细胞标记 OX8。通过对表现出抗原特异性增殖反应的细胞进行有限稀释分析，对MBP和TT反应的细胞的平均频率为3.36±。 2.4 和 7.60.+-。每 104 人分别为 4.1 人。在引流淋巴结中，对MBP和TT反应的细胞频率为2.24.+-。 1.7 和 2.69.+-。 2.5/104。在临床 EAE 的相对早期阶段，MBP 反应性 T 细胞仅占可从脊髓中分离的细胞的一小部分；对与 EAE 发病机制无关的蛋白质抗原产生反应的淋巴细胞的数量相当。这一发现表明，大多数这些细胞的积累是由于 MBP 反应性淋巴细胞不具有特异性的机制造成的。
Lymphocytes were isolated from the spinal cord and draining lymph nodes of Lewis rats with acute experimental allergic encephalomyelitis (EAE) 12 days after immunization with myelin basic protein (MBP) and tetanus toxoid (TT). An average of 8.0 .+-. 2.0 .times. 106 cells was obtained from the spinal cord. Of these 71.1 .+-. 8.6% expressed the helper T-cell marker W3/25 and 14.8 .+-. 6.2% expressed the killer/suppressor-T-cell marker OX8. By limiting dilution analysis of cells exhibiting an antigen-specific proliferative response, the average frequencies of cells reactive to MBP and TT were 3.36 .+-. 2.4 and 7.60 .+-. 4.1 per 104, respectively. In the draining lymph nodes, the frequencies of cells reactive to MBP and TT were 2.24 .+-. 1.7 and 2.69 .+-. 2.5 per 104. At a relatively early stage of clinical EAE, MBP-reactive T cells comprise only a small minority of the cells which can be isolated from the spinal cord; lymphocytes reactive to a protein antigen irrelevant to EAE pathogenesis are present in comparable numbers. This finding suggests that most of these cells accumulate as a result of mechanisms not specific for MBP-reactive lymphocytes.