Tight junction structure and ZO-1 content are identical in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance.
Tight junction structure and ZO-1 content are identical in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance.
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DOI：
10.1083/jcb.107.6.2401
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发表时间：
1988-12
期刊：
影响因子：
--
通讯作者：
Mooseker MS
中科院分区：
文献类型：
--
作者：
Stevenson BR;Anderson JM;Goodenough DA;Mooseker MS
紧密连接通透性的连接结构和组成的关系进行了研究，使用两种菌株的Madin-Darby犬肾（MDCK）细胞（I和II），其差异大于30倍的跨上皮电阻。在大多数条件下，该参数主要由细胞旁（因此是连接）渗透性决定。当这两种细胞株在可渗透的过滤器支持物上生长时，它们形成单层，单层的连接/面积的线性量相等。这些单层的超薄切片EM超微结构分析显示，在整体细胞形态或紧密连接组织没有差异。冷冻断裂制剂的形态计量学分析表明，这两种细胞株的紧密连接的连接纤维的数量和密度相似。根据该冷冻断裂数据和已发表的结构-功能公式预测两种菌株的跨上皮抗性（Claude，P. 1978，J. Memb. 39：219- 232）产生的值（I = 26.5 Ω/cm 2，II = 35.7 Ω/cm 2）显著低于观察到的值（I = 2，500 - 5，000 Ω/cm 2，II = 50-70 Ω/cm 2）。与这些结构研究一致，ZO-1（一种仅定位于紧密连接的多肽）的分布和细胞含量的比较显示，ZO-1的定位或每微米连接的ZO-1量均无显著差异（I = 1，415 +/- 101分子/微米，II = 1，514 +/- 215分子/微米）。
The relationship of tight junction permeability to junction structure and composition was examined using two strains of Madin-Darby canine kidney (MDCK) cells (I and II) which differ greater than 30-fold in transepithelial resistance. This parameter is largely determined by paracellular, and hence junctional, permeability under most conditions. When these two strains of cells were grown on permeable filter supports, they formed monolayers with equivalent linear amounts of junction/area of monolayer. Ultrastructural analysis of these monolayers by thin section EM revealed no differences in overall cellular morphology or in tight junction organization. Morphometric analysis of freeze-fractured preparations indicated that the tight junctions of these two cell strains were similar in both number and density of junctional fibrils. Prediction of transepithelial resistance for the two strains from this freeze-fracture data and a published structure-function formulation (Claude, P. 1978, J. Memb. Biol. 39:219- 232) yielded values (I = 26.5 omega/cm2, II = 35.7 omega/cm2) that were significantly lower than those observed (I = 2,500-5,000 omega/cm2, II = 50-70 omega/cm2). Consistent with these structural studies, a comparison of the distribution and cellular content of ZO-1, a polypeptide localized exclusively to the tight junction, revealed no significant differences in either the localization of ZO-1 or the amount of ZO-1 per micron of junction (I = 1,415 +/- 101 molecules/micron, II = 1,514 +/- 215 molecules/micron).