Poly(lactic acid/caprolactone) bilayer membrane blocks bacterial penetration
Poly(lactic acid/caprolactone) bilayer membrane blocks bacterial penetration
复制标题
DOI：
10.1111/jre.12980
复制
发表时间：
2022-02-25
影响因子：
3.5
通讯作者：
Imazato, Satoshi
中科院分区：
文献类型：
--
作者：
Abe, Gabriela L.;Tsuboi, Ririko;Imazato, Satoshi
背景与目的如果手术部位发生细菌感染，引导组织再生（GTR）或引导骨再生（GBR）手术的临床效果可能会受到影响。膜暴露是细菌感染发病的原因之一。在此之前，我们制备了一种由多孔层和致密层组成的聚乳酸/己内酯（PLCL）双层膜。致密层可作为对结缔组织和上皮细胞的屏障，我们假设它也可能是对细菌细胞的有效屏障。本研究的目的是评估PLCL双层膜阻断细菌细胞渗透的能力，这将有助于预防术后感染。方法对人唾液中的牙龈卟啉单胞菌、变形链球菌和多种细菌进行检测。将细菌直接播种在PLCL双层膜的致密层上，并评估细菌对膜的粘附以及对膜结构的渗透。通过在6、24和72 h形成的菌落数量来评估细菌的粘附性，并在24和72 h使用扫描电镜观察渗透情况。用市售的由聚（乳酸-羟基乙酸）或I型胶原组成的膜作为对照。结果从人唾液中获得的牙龈假单胞菌、变形链球菌和多菌种细菌在孵育6 h后就粘附在所有细胞膜上。然而，在所有实验期间，与对照组相比，PLCL双层膜的贴壁细胞较少。PLCL膜能够阻断细菌的渗透，结构中未观察到细菌细胞。相比之下，细菌穿透了两种对照膜，并在孵育72小时后在80 μ m的深度观察到。结论膜特性可能影响细菌定植的发生。PLCL膜具有减少细菌粘附和阻断细菌渗透的特性，这些特性有助于再生治疗的良好结果。在GTR/GBR手术部位发生膜暴露时，具有有效屏障功能的膜，如PLCL双层膜，可以简化GTR/GBR并发症的处理。
Background and Objective The clinical outcomes of guided tissue regeneration (GTR) or guided bone regeneration (GBR) procedures can be impaired if a bacterial infection develops at the surgical site. Membrane exposure is one of the causes of the onset of bacterial infection. Previously, we have fabricated a poly(lactic acid/caprolactone) (PLCL) bilayer membrane composed of a porous layer and a compact layer. The compact layer acts as a barrier against connective tissue and epithelial cells, and we hypothesized that it could also be an effective barrier against bacterial cells. The objective of this study was to evaluate the ability of the PLCL bilayer membrane to block bacterial cell penetration, which would be useful for preventing postoperative infections. Methods Porphyromonas gingivalis, Streptococcus mutans, and multispecies bacteria collected from human saliva were used in this study. Bacteria were seeded directly on the compact layer of a PLCL bilayer membrane, and bacterial adhesion to the membrane, as well as penetration into the membrane's structure, were assessed. Bacterial adhesion was evaluated by the number of colonies formed at 6, 24, and 72 h, and penetration was observed using a scanning electron microscope at 24 and 72 h. Commercially available membranes, composed of poly(lactic-co-glycolic acid) or type I collagen, were used as controls. Results P. gingivalis, S. mutans, and the multispecies bacteria obtained from human saliva adhered onto all the membranes after only 6 h of incubation. However, fewer adherent cells were observed for the PLCL bilayer membrane compared with the controls for all experimental periods. The PLCL membrane was capable of blocking bacterial penetration, and no bacterial cells were observed in the structure. In contrast, bacteria penetrated both the control membranes and were observed at depths of up to 80 mu m after 72 h of incubation. Conclusion Membrane characteristics may influence how bacterial colonization occurs. The PLCL membrane had reduced bacterial adhesion and blocked bacterial penetration, and these characteristics could contribute to a favorable outcome for regenerative treatments. In the event of membrane exposure at GTR/GBR surgical sites, membranes with an efficient barrier function, such as the PLCL bilayer membrane, could simplify the management of GTR/GBR complications.