NEURALLY EXPRESSED DROSOPHILA GENES ENCODING HOMOLOGS OF THE NSF AND SNAP SECRETORY PROTEINS
NEURALLY EXPRESSED DROSOPHILA GENES ENCODING HOMOLOGS OF THE NSF AND SNAP SECRETORY PROTEINS
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DOI：
10.1073/pnas.91.12.5715
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发表时间：
1994-06-07
影响因子：
11.1
通讯作者：
GANETZKY, B
中科院分区：
文献类型：
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作者：
ORDWAY, RW;PALLANCK, L;GANETZKY, B
几条线的调查，现在已经收敛到表明，神经递质释放装置是由组装的胞质蛋白质与突触囊泡和突触前终末膜的蛋白质。我们正在进行遗传学的方法在果蝇调查的功能，两种类型的胞质蛋白质认为在这个复杂的功能：N-乙基马来酰亚胺敏感的融合蛋白（NSF）和可溶性NSF附着蛋白（SNAPs）。我们已经鉴定了脊椎动物和酵母NSF和SNAP基因的果蝇同源物。这两个果蝇基因编码的多肽与脊椎动物的多肽非常相似，并在神经系统中表达;这两个基因似乎都不属于密切相关的果蝇基因家族。这些结果表明，果蝇NSF和SNAP基因是神经递质释放突变分析的优秀候选者。
Several lines of investigation have now converged to indicate that the neurotransmitter release apparatus is formed by assembly of cytosolic proteins with proteins of the synaptic vesicle and presynaptic terminal membranes. We are undertaking a genetic approach in Drosophila melanogaster to investigate the functions of two types of cytosolic proteins thought to function in this complex: N-ethylmaleimide-sensitive fusion protein (NSF) and the soluble NSF attachment proteins (SNAPs). We have identified Drosophila homologs of the vertebrate and yeast NSF and SNAP genes. Both Drosophila genes encode polypeptides that closely resemble their vertebrate counterparts and are expressed in the nervous system; neither appears to be in a family of closely related Drosophila genes. These results indicate that the Drosophila NSF and SNAP genes are excellent candidates for mutational analysis of neurotransmitter release.