PROGRESSIVE INCORPORATION OF PROPIDIUM IODIDE IN CULTURED MOUSE NEURONS CORRELATES WITH DECLINING ELECTROPHYSIOLOGICAL STATUS - A FLUORESCENCE SCALE OF MEMBRANE INTEGRITY
PROGRESSIVE INCORPORATION OF PROPIDIUM IODIDE IN CULTURED MOUSE NEURONS CORRELATES WITH DECLINING ELECTROPHYSIOLOGICAL STATUS - A FLUORESCENCE SCALE OF MEMBRANE INTEGRITY
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DOI：
10.1016/0165-0270(90)90007-3
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发表时间：
1990-01-01
影响因子：
3
通讯作者：
MADISON, RD
中科院分区：
文献类型：
--
作者：
MACKLIS, JD;MADISON, RD
我们描述了一个直观的测定神经元的电生理状态与培养的神经元，碘化丙啶（PI）的基础上排除完整的细胞膜。我们使用这种与核酸结合的荧光染料，其浓度适合长期暴露于神经元而无毒性。我们与逐步丧失静息膜电位和逐步无法产生刺激的动作电位培养的小鼠背根神经节神经元与PI的掺入增加。使用标准荧光显微镜，用于测量PI结合的评分系统是快速的且高度可重复的。应用存在于体外神经元毒性、存活和电生理学的研究。
We describe a visual assay of neuronal electrophysiologic status for use with cultured neurons, based on the exclusion of propidium iodide (PI) by intact cellular membranes. We use this fluorescent dye, which binds to nucleic acids, at concentrations suitable for long-term exposure to neurons without toxicity. We correlate the progressive loss of resting membrane potential and the progressive inability to generate stimulated action potentials by cultured mouse dorsal root ganglion neurons with increasing incorporation of PI. The scoring system used to gauge incorportion of PI is rapid and highly reproducible using a standard fluorescence microscope. Applications exist for studies of neuronal toxicity, survival and electrophysiology in vitro.