Antioxidant and Antiatherogenic Properties of Phenolic Acid and Flavonol Fractions of Fruits of 'Amari' and 'Hallawi' Date (Phoenix dactylifera L.) Varieties
Antioxidant and Antiatherogenic Properties of Phenolic Acid and Flavonol Fractions of Fruits of 'Amari' and 'Hallawi' Date (Phoenix dactylifera L.) Varieties
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DOI：
10.1021/jf506094r
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发表时间：
2015-04-01
影响因子：
6.1
通讯作者：
Aviram, Michael
中科院分区：
文献类型：
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作者：
Borochov-Neori, Hamutal;Judeinstein, Sylvie;Aviram, Michael
枣（凤凰）在体外检测果实酚酸或黄酮醇级分的抗氧化和抗动脉粥样硬化性质。采用C18固相萃取技术，从两个枣品种‘Amari’和‘Hallawi’中分别提取出各亚类的两个组分。通过RP-HPLC分析了各组分的酚类成分，并测试了铁还原抗氧化能力、自由基清除能力、对Cu 2+诱导的LDL氧化的抑制以及对HDL介导的胆固醇从巨噬细胞流出的增强。所有四个馏分表现出可变的能力，以减少铁离子，自由基，并抑制LDL氧化。与酚酸组分相比，黄酮醇组分是相当好的LDL氧化抑制剂，IC 50分别为9-31 nmol GAE mL（-1）和85-116 nmol GAE mL（-1）。只有黄酮醇馏分刺激胆固醇从巨噬细胞中清除。在每个亚组内，所有活动的水平随馏分组成而变化。结果表明枣酚类化合物具有很强的构效关系，并确定枣黄酮醇类化合物为潜在的抗动脉粥样硬化生物活性物质。
Date (Phoenix dactylifera L.) fruit phenolic-acid or flavonol fractions were examined in vitro for antioxidant and antiatherogenic properties. Two fractions of each subgroup were prepared from two date varieties, 'Amari' and 'Hallawi', by solid phase extraction on C18. The fractions were analyzed for phenolics composition by RP-HPLC and tested for ferric-reducing antioxidant power, free radical scavenging capacity, inhibition of Cu2+-induced LDL oxidation, and enhancement of HDL-mediated cholesterol efflux from macrophages. All four fractions exhibited variable capacities to reduce ferric ions, scavenge radicals, and inhibit LDL oxidation. Flavonol fractions were considerably better inhibitors of LDL oxidation compared to phenolic acid fractions, with IC50's of 9-31 nmol GAE mL(-1) compared to 85-116 nmol GAE mL(-1), respectively. Only the flavonol fractions stimulated cholesterol removal from macrophages. Within each subgroup, the levels of all the activities varied with fraction composition. The results demonstrated strong structure-activity relationships for date phenolics and identified date flavonols as potential antiatherogenic bioactives.