Differential induction of three pathogenesis-related genes, PR10, PR1b and PR5 by the ethylene generator ethephon under light and dark in rice (Oryza sativa L.) seedlings
Differential induction of three pathogenesis-related genes, PR10, PR1b and PR5 by the ethylene generator ethephon under light and dark in rice (Oryza sativa L.) seedlings
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DOI：
10.1078/0176-1617-00186
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发表时间：
2001-01-01
影响因子：
4.3
通讯作者：
Jwa, NS
中科院分区：
文献类型：
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作者：
Agrawal, GK;Rakwal, R;Jwa, NS
乙烯主要在双子叶植物中参与触发致病相关（PR）基因的表达；然而，水稻（Oryza sativa L.）作为一种单子叶植物和谷类作物属的典范，其PR基因的鉴定和表征却很少受到关注。在这里，我们证明乙烯诱导了至少三个重要的水稻PR基因，PR10， PR1碱性（PR1b）和PR5基因。经乙烯发生器乙烯利（ET）处理后，日本赤竹幼苗以光、时间和剂量依赖的方式生长。这些PR基因的诱导被真核细胞质蛋白质合成抑制剂环己亚胺（CHX）部分阻断，这表明细胞质从头合成蛋白质参与了它们的诱导。这些结果清楚地表明乙烯在水稻PR基因诱导中的动态作用。
Ethylene has been shown to be involved in triggering pathogenesis-related (PR) gene expression mainly in dicotyledonous species; however, less attention has been devoted identifying and characterizing PR genes in rice (Oryza sativa L.), a monocot and a model of cereal crop genera. Here, we demonstrate that ethylene induces at least three important rice PR genes, the PR10, PR1 basic (PR1b), and PR5 genes in rice (cv. Nipponbare) seedling lear, upon treatment with the ethylene generator, ethephon (ET), in a light-, time- and dose-dependent manner. Induction of these PR genes was partially blocked by cycloheximide (CHX), a eukaryotic cytoplasmic protein synthesis inhibitor, which indicates an involvement of cytoplasmic de novo protein synthesis in their induction. These results clearly indicate a dynamic role for ethylene in PR genes induction in rice.