Clearance of apoptotic cells is not impaired in mouse embryos deficient in class A scavenger receptor types I and II (CD204)
Clearance of apoptotic cells is not impaired in mouse embryos deficient in class A scavenger receptor types I and II (CD204)
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DOI：
10.1002/dvdy.20206
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发表时间：
2005-01-01
影响因子：
2.5
通讯作者：
Takeya, M
中科院分区：
文献类型：
--
作者：
Komohara, Y;Terasaki, Y;Takeya, M
凋亡细胞的清除是维持胚胎正常形态发生的重要机制。A类清道夫受体I， II型（CD204）是巨噬细胞表达的主要受体之一，是一种积极参与凋亡细胞识别和摄取的受体。为了阐明CD204在胚胎形态发生中的作用，我们使用缺乏CD204的小鼠胚胎进行了免疫组织化学和免疫电镜研究。在对照组小鼠中，几乎所有巨噬细胞从胚胎9.5天（E9.5）开始表达CD204。E13.5趾间区吞噬死细胞的吞噬细胞强烈表达CD204，表明CD204积极参与凋亡细胞的清除。然而，CD204并不是胚胎清除凋亡细胞所必需的，因为缺乏CD204的胚胎发育正常，在足底重塑中没有任何迟缓。在CD204缺失的胎儿巨噬细胞中，CD36的上调表明CD36替代了CD204的功能。我们还发现间充质细胞经常吞噬凋亡细胞，特别是在胚胎早期。这些数据表明，CD204部分而非主要参与胚胎发生过程中凋亡细胞的清除。在胚胎早期发育过程中，间充质细胞而非巨噬细胞在凋亡细胞清除中起主要作用。(C) 2004 Wiley-Liss, Inc。
Elimination of apoptotic cells is an important mechanism to maintain proper embryonal morphogenesis. The class A scavenger receptor type I, II (CD204), one of the major receptors expressed on macrophages, is a receptor actively involved in recognition and ingestion of apoptotic cells. To clarify the role of CD204 in embryonic morphogenesis, we performed immunohistochemical and immunoelectron microscopic studies using CD204-deficient mouse embryos. In control mice, almost all macrophages expressed CD204 from embryonic day 9.5 (E9.5). Phagocytes engulfing dead cells in the E13.5 interdigit region showed strong expression of CD204, indicating that CD204 was actively involved in apoptotic cell clearance. However, CD204 is not essential for the embryonic clearance of apoptotic cells, because CD204-deficient embryos developed normally without any retardation in footplate remodeling. Up-regulation of CD36 in CD204-deficient fetal macrophages suggested that CD36 substitutes for CD204 function. We also found that mesenchymal cells frequently engulfed apoptotic cells especially in early embryonal stages. These data suggest that CD204 is partially but not essentially involved in apoptotic cell clearance in embryogenesis. During early embryonal development, mesenchymal cells, rather than macrophages, play a major role in apoptotic cell clearance. (C) 2004 Wiley-Liss, Inc.