Human stanniocalcin: A possible hormonal regulator of mineral metabolism
Human stanniocalcin: A possible hormonal regulator of mineral metabolism
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DOI：
10.1073/pnas.93.5.1792
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发表时间：
1996-03-05
影响因子：
11.1
通讯作者：
Wagner, GF
中科院分区：
文献类型：
--
作者：
Olsen, HS;Cepeda, MA;Wagner, GF
我们已经分离出一个编码STC的人类cDNA克隆，STC是一种钙和磷酸盐调节激素，最初在鱼类中被描述为具有预防高钙血症的功能。STC具有独特的氨基酸序列，直到现在，它仍然是为数不多的从未在高等脊椎动物中描述过的多肽激素之一。人类STC （hSTC）全长247个氨基酸，与鱼类STC的氨基酸序列相似性为73%。重组hSTC的多克隆抗体定位于肾小管中不同类型的细胞，提示肾脏可能是合成部位。重组hSTC可抑制鱼鳃钙转运，刺激大鼠肾磷重吸收。有证据表明，哺乳动物的STC和鱼类的STC一样，是矿物质体内平衡的调节器。
We have isolated a human cDNA clone encoding the mammalian homolog of stanniocalcin (STC), a calcium- and phosphate-regulating hormone that was first described in fishes where it functions in preventing hypercalcemia. STC has a unique amino acid sequence and, until now, has remained one of the few polypeptide hormones never described in higher vertebrates. Human STC (hSTC) was found to be 247 amino acids long and to share 73% amino acid sequence similarity with fish STC. Polyclonal antibodies to recombinant hSTC localized to a distinct cell type in the nephron tubule, suggesting kidney as a possible site of synthesis. Recombinant hSTC inhibited the gill transport of calcium when administered to fish and stimulated renal phosphate reabsorption in the rat. The evidence suggests that mammalian STC, like its piscine counterpart, is a regulator of mineral homeostasis.