INVIVO AND INVITRO BINDING OF BENZENE TO NUCLEIC-ACIDS AND PROTEINS OF VARIOUS RAT AND MOUSE ORGANS
INVIVO AND INVITRO BINDING OF BENZENE TO NUCLEIC-ACIDS AND PROTEINS OF VARIOUS RAT AND MOUSE ORGANS
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DOI：
10.1016/0304-3835(85)90071-0
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发表时间：
1985-01-01
期刊：
影响因子：
9.7
通讯作者：
PRODI, G
中科院分区：
文献类型：
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作者：
ARFELLINI, G;GRILLI, S;PRODI, G
苯与大鼠和小鼠体内各种器官的大分子结合。RNA和蛋白质的标记高于DNA标记（1个数量级），DNA标记在许多器官（肝、脾、骨髓和肾）中较低，在肺中可忽略不计;大鼠和小鼠器官的标记之间没有差异，共价结合指数（CBI）值约为10，即归类为弱引发剂的遗传毒性致癌物的典型值。在体外，苯与核酸和蛋白质的结合是由肝微粒体介导的，而不是由肾、脾和肺的微粒体或任何器官的胞质溶胶介导的。核酸结合可通过苯巴比妥（PB）预处理诱导，并在SKF 525-A、细胞质和/或GSH或热灭活微粒体存在下抑制。外源DNA的标记较低，并且在大鼠或小鼠微粒体存在下相似，与体内测得的与DNA的低相互作用一致。
Benzene binds to macromolecules of various organs in the rat and mouse in vivo. Labelling of RNA and proteins is higher (1 order of magnitude) than DNA labelling, which is low in many organs (liver, spleen, bone marrow and kidney), and negligible in lung; no difference between labelling of rat and mouse organs was found. The covalent binding index (CBI) value was about 10, i.e. typical of genotoxic carcinogens classified as weak initiators. In vitro binding of benzene to nucleic acids and proteins is mediated by hepatic microsomes, but not by microsomes from kidney, spleen and lung, or by cytosol from whatever organ. Nucleic acid binding can be induced by pretreatment with phenobarbitone (PB) and suppressed in the presence of SKF 525-A, of cytosol and/or GSH or of heat-inactivated microsomes. Labelling of exogenous DNA is low and is similar in the presence of rat or mouse microsomes in agreement with the low interaction with DNA measured in vivo.