Corneal innervation as a window to peripheral neuropathies
Corneal innervation as a window to peripheral neuropathies
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DOI：
10.1016/j.exer.2013.05.016
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发表时间：
2013-08-01
影响因子：
3.4
通讯作者：
Oaklander, Anne Louise
中科院分区：
文献类型：
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作者：
Ferrari, Giulio;Nalassamy, Nambi;Oaklander, Anne Louise
角膜接受身体最密集的感觉神经支配，它完全来自小纤维伤害性(痛感)神经元。这些部位与腿部皮肤相似，后者是神经诊断性皮肤活检的标准部位，用于诊断小纤维外周多发性神经病。许多癌症化疗药物会导致剂量相关、治疗受限、感官主导的多发性神经病。由于角膜神经支配可以非侵入性地检测到，因此它是皮肤活检测量的潜在替代生物标志物。因此，我们比较了接受神经毒性化疗的小鼠的后掌皮肤神经支配和角膜神经支配。给C57/BL6小鼠注射紫杉醇(0、5、10或20 mg/kg)，并对死后角膜和皮肤活检进行免疫标记，以揭示和允许神经支配的数量。两种组织均表现出剂量依赖、高度相关(r=0.66)的神经纤维损伤。这些结果表明，角膜神经的定量可能为皮肤周围神经支配提供一个有用的替代标志物。(C)2013爱思唯尔有限公司。保留所有权利。
The cornea receives the densest sensory innervation of the body, which is exclusively from small-fiber nociceptive (pain-sensing) neurons. These are similar to those in the skin of the legs, the standard location for neurodiagnostic skin biopsies used to diagnose small-fiber peripheral polyneuropathies. Many cancer chemotherapy agents cause dose-related, therapy-limiting, sensory-predominant polyneuropathy. Because corneal innervation can be detected non-invasively, it is a potential surrogate biomarker for skin biopsy measurements. Therefore, we compared hindpaw-skin and cornea innervation in mice treated with neurotoxic chemotherapy. Paclitaxel (0, 5, 10, or 20 mg/kg) was administered to C57/Bl6 mice and peri-mortem cornea and skin biopsies were immunolabeled to reveal and permit quantitation of innervation. Both tissues demonstrated dose-dependent, highly correlated (r = 0.66) nerve fiber damage. These findings suggest that the quantification of corneal nerves may provide a useful surrogate marker for skin peripheral innervation. (C) 2013 Elsevier Ltd. All rights reserved.