Identification and phylogenetic analysis of late embryogenesis abundant proteins family in tomato (Solanum lycopersicum)
Identification and phylogenetic analysis of late embryogenesis abundant proteins family in tomato (Solanum lycopersicum)
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DOI：
10.1007/s00425-014-2215-y
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发表时间：
2015-03-01
期刊：
影响因子：
4.3
通讯作者：
Li, Xiang
中科院分区：
文献类型：
--
作者：
Cao, Jun;Li, Xiang
本研究对LEA基因家族进行了比较基因组学分析，这可能为其未来的功能研究提供有价值的信息。LEA蛋白是一组在许多生物体中因细胞脱水而积累的蛋白质。在这里，我们在番茄中鉴定了27个LEA基因。在系统发育、基因结构和基序组成之间发现了很强的相关性。预测的sllea基因在它们的染色体中是非随机分布的，分段和串联复制可能对它们的扩展很重要。在启动子区域上游的1000bp序列中也发现了许多可能介导非生物胁迫转录的顺式元件。一个额外的基因内重组在slleA基因的进化中发挥了重要作用。选择分析还发现了一些重要的特定部位的限制因素，这些限制因素对大多数LEA并列基因的进化起作用。利用基因芯片数据和实时定量聚合酶链式反应进行的基因表达分析表明，slleA基因在多种组织中广泛表达，少数成员对某些非生物胁迫做出反应。我们的研究为番茄LEA基因的研究提供了有用的信息，并将有助于进一步鉴定这些基因以更好地了解它们的功能。
This study provided a comparative genomic analysis of the LEA gene family, and these may provide valuable information for their functional investigations in the future.Late embryogenesis abundant (LEA) proteins are a group of proteins that accumulate in response to cellular dehydration in many organisms. Here, we identified 27 LEA genes in tomato. A strong correlation between phylogeny, gene structure, and motif composition was found. The predicted SlLEA genes were non-randomly distributed within their chromosomes, and segmental and tandem duplications were probably important for their expansion. Many cis-elements potentially mediating transcription in response to abiotic stress were also found in the 1,000 bp upstream sequence of the promoter region. An additional intragenic recombination played an important role in the evolution of SlLEA genes. Selection analysis also identified some significant site-specific constraints that acted on the evolution of most LEA paralogs. Expression analysis using both microarray data and quantitative real-time PCR indicated that SlLEA genes were widely expressed in various tissues, and that a few members responded to some abiotic stresses. Our study provides useful information on the LEA genes in tomato and will facilitate their further characterization to better understand their functions.