The Role of Surface Nanotopography and Chemistry on Primary Neutrophil and Macrophage Cellular Responses
The Role of Surface Nanotopography and Chemistry on Primary Neutrophil and Macrophage Cellular Responses
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DOI：
10.1002/adhm.201500845
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发表时间：
2016-04-20
影响因子：
10
通讯作者：
Vasilev, Krasimir
中科院分区：
文献类型：
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作者：
Christo, Susan N.;Bachhuka, Akash;Vasilev, Krasimir
用于增强、替代或恢复生物功能的合成材料可能会受到它们所引起的宿主免疫反应的影响。表面修饰作为一种调节宿主对合成材料的反应的工具已经引起了人们的广泛关注；然而，表面纳米形貌与化学相结合如何影响免疫效应细胞的反应仍然知之甚少。为了解决这一悬而未决的问题，已经产生了一组独特的模型表面，其表面纳米形貌可控在16、38和68 nm的范围内。提供了富含胺、羧基或甲基的定制最外表面化学。这些特性的组合产生了12种表面类型，这些类型需要进行功能分析来评估关键的免疫效应细胞，即体外原代中性粒细胞和巨噬细胞的反应。数据表明，表面纳米拓扑术导致原代中性粒细胞产生的基质金属蛋白酶-9增加，而原代巨噬细胞的促炎细胞因子分泌减少。总而言之，这些结果首次直接比较了表面纳米拓扑学和化学之间的协同作用的免疫调节效果。
Synthetic materials employed for enhancing, replacing, or restoring biological functionality may be compromised by the host immune responses that they evoke. Surface modification has attracted substantial attention as a tool to modulate the host response to synthetic materials; however, how surface nanotopography combined with chemistry affects immune effector cell responses is still poorly understood. To address this open question, a unique set of model surfaces with controlled surface nanotopography in the range of 16, 38, and 68 nm has been generated. Tailored outermost surface chemistry that was amine, carboxyl, or methyl group rich has been provided. The combinations of these properties yield 12 surface types that are subject to functional assays assessing key immune effector cells, namely, primary neutrophil and macrophage responses in vitro. The data demonstrate that surface nanotopography leads to enhanced matrix metalloproteinase-9 production from primary neutrophils, and a decrease in pro-inflammatory cytokine secretion from primary macrophages. Together, these results are the first to directly compare the immunomodulatory effects of the cooperative interplay between surface nanotopography and chemistry.