Rapid Specific and Label-Free Detection of Porcine Rotavirus Using Photonic Crystal Biosensors
Rapid Specific and Label-Free Detection of Porcine Rotavirus Using Photonic Crystal Biosensors
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DOI：
10.1109/jsen.2009.2014427
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发表时间：
2009-04-01
影响因子：
4.3
通讯作者：
Cunningham, Brian T.
中科院分区：
文献类型：
--
作者：
Pineda, Maria Fernanda;Chan, Leo Li-Ying;Cunningham, Brian T.
证明了一种使用光子晶体生物传感器直接检测完整活轮状病毒的快速、无标记的检测方法。本文介绍了该生物传感器的设计和操作，抗轮状病毒抗体的表面功能化，检测灵敏度的表征，以及对非靶标病毒颗粒的特异性展示。对部分处理的水样进行30分钟的检测，在不使用任何外部试剂的情况下，检测灵敏度为36个病毒焦点形成单位。这种敏感性可与商用的酶联免疫吸附试验相媲美。这些结果表明，光子晶体生物传感器可用于环境水资源病毒污染的实时监测。
A rapid, label-free assay using photonic crystal biosensors for the direct detection of intact viable rotaviruses is demonstrated. This paper describes the design and operation of the biosensor, surface functionalization with anti-rotavirus antibody, characterization of the detection sensitivity, and demonstration of specificity with respect to non-target virus particles. A 30-min assay of a partially processed water sample yielded a detection sensitivity of 36 virus focus forming units without the use of any external reagents. This sensitivity is comparable to commercial enzyme-linked immunosorbent assays. These results suggest photonic crystal biosensors may be useful for real-time monitoring of virus contamination of environmental water resources.