RAPID SELECTION OF CELL SUBPOPULATION-SPECIFIC HUMAN MONOCLONAL-ANTIBODIES FROM A SYNTHETIC PHAGE ANTIBODY LIBRARY
RAPID SELECTION OF CELL SUBPOPULATION-SPECIFIC HUMAN MONOCLONAL-ANTIBODIES FROM A SYNTHETIC PHAGE ANTIBODY LIBRARY
复制标题
DOI：
10.1073/pnas.92.9.3938
复制
发表时间：
1995-04-25
影响因子：
11.1
通讯作者：
LOGTENBERG, T
中科院分区：
文献类型：
--
作者：
DEKRUIF, J;TERSTAPPEN, L;LOGTENBERG, T
用半合成噬菌体抗体库和荧光标记的CD3和CD20抗体孵育外周血白细胞，通过流式细胞术分离对血液白细胞亚群特异性的人单链Fv抗体。分离的噬菌体抗体显示出与用于选择的亚群的排他性结合，或显示出与混合物中的其他细胞的受限群体的额外结合。至少有两种噬菌体抗体显示出迄今未知的B系细胞染色模式。这种方法提供了一种消减方法，以快速获得针对已知和新的表面抗原的人抗体，其呈天然构型，在表型确定的细胞亚群上表达。这种方法不依赖于免疫程序或重复构建噬菌体抗体库的必要性。
Peripheral blood leukocytes incubated with a semisynthetic phage antibody library and fluorochrome-labeled CD3 and CD20 antibodies were used to isolate human single-chain Fv antibodies specific for subsets of blood leukocytes by flow cytometry. Isolated phage antibodies showed exclusive binding to the subpopulation used for selection or displayed additional binding to a restricted population of other cells in the mixture. At least two phage antibodies appeared to display hitherto-unknown staining patterns of B-lineage cells, This approach provides a subtractive procedure to rapidly obtain human antibodies against known and novel surface antigens ill their native configuration, expressed on phenotypically defined subpopulations of cells. This approach does not depend on immunization procedures or the necessity to repeatedly construct phage antibody libraries.