Enhanced virus recovery from municipal sewage sludge with a combination of enzyme and cation exchange resin.
Enhanced virus recovery from municipal sewage sludge with a combination of enzyme and cation exchange resin.
复制标题
利用酶和阳离子交换树脂的组合增强城市污水污泥中的病毒回收率。
DOI：
10.2166/wst.2001.0075
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
T. Omura
中科院分区：
文献类型：
--
作者：
D. Sano;K. Fukushi;K. Yano;Y. Yoshida;T. Omura
由于污泥中的病毒被污泥固体牢牢捕获，因此污泥中的病毒计数存在很大困难。为了确定污泥中病毒的精确数量，开发了一种结合酶和阳离子交换树脂（CER）的增强病毒回收方法。将测试病毒接种到从城市污水处理厂获得的样品污泥中，并将污泥与各种洗脱液一起孵育。然后通过空斑测定技术测量液相中洗脱的病毒的量。使用仅含有水的洗脱液，CER和CER与酶的病毒回收率分别为0%、19%和39%。而传统的USEPA方法显示出21%的病毒回收率。此外，由含有CER和溶菌酶的洗脱液洗脱的病毒不仅包括表面附着的病毒，而且包括固体包埋的病毒。
There is a great difficulty in virus enumeration in sewage sludge because viruses in sludge are firmly captured by sludge solids. In order to determine the precise number of viruses in sludge, an enhanced virus recovery method with a combination of an enzyme and a cation exchange resin (CER) was developed. Test viruses were seeded to a sample sludge obtained from a municipal wastewater treatment plant, and the sludge were incubated with various eluents. The quantity of eluted viruses in the liquid phase was then measured by the plaque assay technique. Using the eluent containing only water, CER, and CER with enzyme exhibited 0%, 19% and 39% of virus recovery, respectively. While the conventional USEPA method exhibited a virus recovery of 21%. Furthermore, viruses eluted by the eluent containing the CER and the lysozyme included not only surface-attached viruses but also solids-embedded viruses.