No correlation between the number of fetal nucleated cells and the amount of cell-free fetal DNA in maternal circulation either before or after delivery
No correlation between the number of fetal nucleated cells and the amount of cell-free fetal DNA in maternal circulation either before or after delivery
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DOI：
10.1002/pd.1765
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发表时间：
2007-08-01
影响因子：
3
通讯作者：
Kudo, Yoshiki
中科院分区：
文献类型：
--
作者：
Hyodo, Maki;Samura, Osamu;Kudo, Yoshiki
目的对10例单胎男性孕妇在分娩前后4个时间点的静脉血进行胎儿有核细胞和胎儿游离DNA的平行检测，并探讨两者之间的关系。用孕妇全血计数经双色荧光原位杂交技术处理的具有Y染色体特异性信号的胎儿有核细胞的数目。结果分娩后胎儿有核细胞数量减少，分娩后1个月仍有部分胎儿细胞存在。而胎儿游离DNA在分娩后迅速下降，分娩后第1天即检测不到。胎儿有核细胞的数量与母体循环中胎儿游离DNA的浓度无相关性。结论胎儿游离DNA在分娩后迅速消失，胎儿有核细胞在母体循环中保留时间较长，两者在分娩前后均无相关性。版权所有（c）2007约翰威利父子有限公司。
Objectives We have determined the number of fetal nucleated cells and the concentration of cell-free fetal DNA in parallel in the same maternal blood samples either before or after delivery, and studied the relationship between these two.Methods Venous blood samples were taken at four points around delivery from ten women who had singleton male fetus with informed consent. The number of fetal nucleated cells having a Y chromosome specific signal treated by two-color fluorescence in situ hybridization technique was counted using maternal whole blood. The concentration of sex-determining region Y gene sequence was determined using real-time quantitative PCR.Results The number of fetal nucleated cells decreased after delivery, and some fetal cells were present I month after delivery. While cell-free fetal DNA decreased rapidly after delivery and became undetectable I day after delivery in eight out of ten cases. The number of fetal nucleated cells did not correlate with the concentration of cell-free fetal DNA in maternal circulation.Conclusion The present study demonstrates that cell-free fetal DNA disappears very rapidly after delivery and fetal nucleated cells remain longer in maternal circulation, and that there is no correlation between these two either before or after delivery. Copyright (c) 2007 John Wiley & Sons, Ltd.