SALMONELLA-TYPHIMURIUM BACTEREMIA - ASSOCIATION WITH THE VIRULENCE PLASMID
SALMONELLA-TYPHIMURIUM BACTEREMIA - ASSOCIATION WITH THE VIRULENCE PLASMID
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DOI：
10.1093/infdis/166.3.639
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发表时间：
1992-09-01
影响因子：
6.4
通讯作者：
GUINEY, D
中科院分区：
文献类型：
--
作者：
FIERER, J;KRAUSE, M;GUINEY, D
在少数沙门氏菌血清型中发现的毒力质粒极大地增强了沙门氏菌的肠外生长，并在实验性小鼠感染中将LD 50降低2-5 log。为了确定毒力质粒在人类沙门氏菌菌血症的发病机制中是否重要，检查了来自加州的鼠伤寒沙门氏菌的血液和粪便分离株中是否存在毒力质粒。使用从都柏林沙门氏菌毒力质粒的高度保守的EcoRI片段制备的标记探针进行菌落印迹。共有42%的粪便和76%的血液分离株与探针杂交。这是第一个证据表明，毒力质粒在人类沙门氏菌菌血症的发病机制中是重要的。
Virulence plasmids, which are found in a small number of Salmonella serotypes, greatly enhance the extraintestinal growth of salmonellae and lower the LD50 by 2-5 logs in experimental murine infections. To determine if virulence plasmids are important in the pathogenesis of Salmonella bacteremia in humans, blood and fecal isolates of Salmonella typhimurium from California were examined for the presence of a virulence plasmid. Colony blots were done using a labeled probe made from the highly conserved EcoRI fragment of the Salmonella dublin virulence plasmid. A total of 42% of the fecal and 76% of the blood isolates hybridized with the probe. This is the first evidence that the virulence plasmid is important in the pathogenesis of Salmonella bacteremia in humans.