Teriparatide promotes healing of critical size femur defect through accelerating angiogenesis and degradation of beta-TCP in OVX osteoporotic rat model
Teriparatide promotes healing of critical size femur defect through accelerating angiogenesis and degradation of beta-TCP in OVX osteoporotic rat model
复制标题
特立帕肽通过加速 OVX 骨质疏松大鼠模型中血管生成和 β-TCP 降解来促进临界尺寸股骨缺损的愈合
DOI：
10.1016/j.biopha.2017.11.141
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发表时间：
2017
影响因子：
7.5
通讯作者：
Yang Lei
中科院分区：
文献类型：
--
作者：
Xie Zhongjie;Weng Sheji;Li Hang;Yu Xia;Lu Shanshan;Huang Kate;Wu Zongyi;Bai Bingli;Boodhun Viraj;Yang Lei
越来越多的证据表明，早期血管生成对损伤的愈合具有重要作用。特立帕肽（PTH）因其对骨的合成代谢活性而被广泛应用，但其促血管生成的能力却知之甚少。在本研究中，我们检测了PTH的血管生成能力及其对去卵巢（OVX）大鼠股骨远端干骺端模型中β-磷酸三钙（β-TCP）降解的影响。在证实OVX模型成功建立后，在OVX大鼠的每个股骨远端钻取临界尺寸的缺损。然后将动物随机分为对照组、β-TCP组和β-TCP+PTH组，β-TCP+PTH组隔日皮下注射特立帕林（30 μg/kg）。股骨手术后四周，每组取五份标本进行Microfil灌注，以显示骨缺损中的血管。剩余大鼠进行显微计算机断层扫描、组织学分析和免疫组化。结果表明，Teriparbine与β-TCP联合使用可促进新生血管形成、β-TCP降解和新骨形成，这可能与早期新生血管形成有关。
Accumulating evidence suggests that early angiogenesis has an important effect on the healing of injury. Teriparatide (PTH) is extensively applied for its potent anabolic activity on bone, while little is known about its angiogenic ability which may facilitate new bone formation. In this study, we tested the angiogenic ability of PTH and its effect on degradation of β-tricalcium phosphate (β-TCP) in an ovariectomized (OVX) rat distal femoral metaphysis model. After successful establishment of the OVX model was confirmed, a critical size defect was drilled into each distal femur of the OVX rats. Afterwards all animals were randomly divided into three groups: control group, group β-TCP and group β-TCP+PTH, then rats of group β-TCP+PTH were injected Teriparatide (30 μg/kg) subcutaneous every other day. Four weeks after femur surgery, five specimens from each group were used for Microfil perfusion to reveal blood vessels in the bone defect. The residual rats were harvested for micro-computed tomography, histological analysis and immunochemistry. The results showed Teriparatide facilitated neovascularization, degradation of β-TCP and new bone formation in combination with β-TCP, which may be relevant to neovascularization in an early phase.