Role of DNA in bacterial aggregation
Role of DNA in bacterial aggregation
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DOI：
10.1007/s00284-008-9166-0
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发表时间：
2008-08-01
影响因子：
2.6
通讯作者：
Xie, Zhi-Xiong
中科院分区：
文献类型：
--
作者：
Liu, Hui-Hui;Yang, Yi-Ran;Xie, Zhi-Xiong
用不同类型的DNA和大肠杆菌确定了DNA在细菌聚集中的作用，这是研究添加的聚合物与细菌聚集和聚合物在细菌表面吸附之间的相关性的一个很好的模型。聚集实验结果表明，胞外DNA确实提高了大肠杆菌的聚集率，但这种作用依赖于DNA的浓度和长度。此外，DNA以一种类型非特异性的方式促进细菌聚集。聚集实验和吸附实验的联合结果进一步表明，DNA促进了大肠杆菌的聚集，同时DNA也吸附到了大肠杆菌上。因此，对DNA促进细菌聚集的可能机制进行了讨论。使用荧光标记的DNA，我们定位了大肠杆菌聚集体内的DNA。随后，DNase I的引入打破了涉及DNA的大肠杆菌聚集体。这些结果表明，DNA作为促进大肠杆菌聚集的分子桥梁发挥作用。
The role of DNA in bacterial aggregation was determined using various types of DNA and Escherichia coli, a good model for investigating the correlation between added polymer and bacterial aggregation and adsorption of polymer to bacterial surfaces. The results of the aggregation assay suggest that extracellular DNA indeed increased the aggregation percentage of E. coli, but this effect was dependent on DNA concentration and length. Moreover, DNA promoted bacterial aggregation in a type-nonspecific way. The combined results of the aggregation assay and the adsorption assay show further that the promotion of E. coli aggregation by DNA occurred along with adsorption of DNA to E. coli. Consequently, the possible mechanisms for DNA-promoted bacterial aggregation are discussed. Using fluorescent-labeled DNA, we mapped DNA within the E. coli aggregates. Subsequently, introduction of DNase I broke up the DNA-involved E. coli aggregates. These results suggest that DNA functions as a molecular bridge to promote E. coli aggregation.