The QDREC web server: determining dose-response characteristics of complex macroparasites in phenotypic drug screens
The QDREC web server: determining dose-response characteristics of complex macroparasites in phenotypic drug screens
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DOI：
10.1093/bioinformatics/btu831
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发表时间：
2015-05-01
期刊：
影响因子：
5.8
通讯作者：
Singh, Rahul
中科院分区：
文献类型：
--
作者：
Asarnow, Daniel;Rojo-Arreola, Liliana;Singh, Rahul
摘要：由蠕虫引起的被忽视的热带病是世界上最贫穷的人最常见的一些感染。病因很复杂，而且不符合标准的分子生物学技术。针对蠕虫的药物筛选通常是表型的，通常涉及药物效果和疗效的人工描述。一个关键的挑战是开发自动化、定量的方法来筛选针对蠕虫疾病的药物。量子剂量-反应计算器(QDREC)是朝着这个方向迈出的重要一步。它可以使用表型屏幕上基于图像的读数自动确定定量剂量-反应特性和半最大有效浓度(EC50)值，从而可以对药物化合物的疗效进行严格的比较。QDREC是在血吸虫病药物筛选的背景下开发和验证的，血吸虫病是最重要的NTD之一。然而，它同样适用于涉及蠕虫和其他复杂寄生虫的一般表型筛选。
A Summary: Neglected tropical diseases (NTDs) caused by helminths constitute some of the most common infections of the world's poorest people. The etiological agents are complex and recalcitrant to standard techniques of molecular biology. Drug screening against helminths has often been phenotypic and typically involves manual description of drug effect and efficacy. A key challenge is to develop automated, quantitative approaches to drug screening against helminth diseases. The quantal dose-response calculator (QDREC) constitutes a significant step in this direction. It can be used to automatically determine quantitative dose-response characteristics and half-maximal effective concentration (EC50) values using image-based readouts from phenotypic screens, thereby allowing rigorous comparisons of the efficacies of drug compounds. QDREC has been developed and validated in the context of drug screening for schistosomiasis, one of the most important NTDs. However, it is equally applicable to general phenotypic screening involving helminths and other complex parasites.