In vitro scratch assay:: a convenient and inexpensive method for analysis of cell migration in vitro
In vitro scratch assay:: a convenient and inexpensive method for analysis of cell migration in vitro
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DOI：
10.1038/nprot.2007.30
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Guan, Jun-Lin
中科院分区：
文献类型：
--
作者：
Liang, Chun-Chi;Park, Ann Y.;Guan, Jun-Lin
体外划痕法是一种简单、低成本、成熟的体外细胞迁移测定方法。基本步骤包括在细胞单层中创建“划痕”，在细胞迁移过程中开始和定期捕获图像以关闭划痕，并比较图像以量化细胞的迁移率。与其他方法相比，体外划痕实验特别适合研究细胞-基质和细胞-细胞相互作用对细胞迁移的影响，在体内模拟伤口愈合过程中的细胞迁移，并且在需要时与活细胞迁移过程中的成像兼容，以监测细胞内事件。除了监测同质细胞群的迁移外，该方法还被用于测量划痕前缘单个细胞的迁移。不考虑转染细胞的时间，体外划痕试验本身通常需要几个小时到一夜之间。
The in vitro scratch assay is an easy, low-cost and well-developed method to measure cell migration in vitro. The basic steps involve creating a "scratch'' in a cell monolayer, capturing the images at the beginning and at regular intervals during cell migration to close the scratch, and comparing the images to quantify the migration rate of the cells. Compared to other methods, the in vitro scratch assay is particularly suitable for studies on the effects of cell - matrix and cell - cell interactions on cell migration, mimic cell migration during wound healing in vivo and are compatible with imaging of live cells during migration to monitor intracellular events if desired. Besides monitoring migration of homogenous cell populations, this method has also been adopted to measure migration of individual cells in the leading edge of the scratch. Not taking into account the time for transfection of cells, in vitro scratch assay per se usually takes from several hours to overnight.