Ethanol production from mahula (Madhuca latifolia L.) flowers using free and immobilized (in Luffa cylindrical L. sponge discs) cells of Zymomonas mobilis MTCC 92
Ethanol production from mahula (Madhuca latifolia L.) flowers using free and immobilized (in Luffa cylindrical L. sponge discs) cells of Zymomonas mobilis MTCC 92
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DOI：
10.1007/s13213-010-0160-y
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发表时间：
2011-09-01
影响因子：
3
通讯作者：
Ray, Ramesh C.
中科院分区：
文献类型：
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作者：
Behera, Shuvashish;Mohanty, Rama C.;Ray, Ramesh C.
采用丝瓜固定化运动发酵单胞菌的新方法。马胡拉发酵生产乙醇的基质研究对花卉进行了调查。丝瓜是热带和亚热带国家盛产的一种葫芦科作物，其干燥的海绵果实被发现是固定微生物细胞的一种有前途的材料。在我们的研究中，这些细胞不仅存活了下来，而且在四个生长周期中都表现出了生理活性，经过96h的发酵，第一、第二、第三和第四个周期的乙醇产量分别为251.1+/-0.08、247.9+/-0.08、243.4+/-0.042和240.71+/-0.033 g/kg花。固定化细胞的乙醇产量比游离细胞高9.2%。固定化丝瓜细胞的乙醇产率(yP/S)为0.439+/-0.004 g/g，体积底物摄取量(Qs)为0.994+/-0.009 g/L/h，最终糖醇转化率(%)为87.8%，分别比游离细胞提高7.06%、2.62%和6.92%。
A novel immobilization method involving Zymomonas mobilis immobilized in a luffa (Luffa cylindrica L.) matrix for ethanol production from mahula (Madhuca latifolia L.) flowers was investigated. The dried spongy fruits of luffa, a cucurbitaceous crop available in abundance in tropical and sub-tropical countries, have been found to be a promising material for immobilizing microbial cells. In our study, the cells not only survived, but they were also active physiologically for four growth cycles, yielding ethanol at 251.1 +/- 0.012, 247.9 +/- 0.08, 243.4 +/- 0.042 and 240.71 +/- 0.033 g/kg flowers in the first, second, third, and fourth cycles, respectively, after 96 h fermentation. Ethanol production by the immobilized cells was 9.2% higher than that by free cells. The ethanol yield (Yp/s), volumetric substrate uptake (Qs), and final sugar to ethanol conversion rate (%) obtained with luffa-immobilized cells of Z. mobilis were 0.439 +/- 0.004 g/g, 0.994 +/- 0.009 g/l/h, and 87.8%, respectively, which were 7.06, 2.62, and 6.92% higher than that of free cells.