Synapse-specific, long-form facilitation of Aplysia sensory to motor synapses: A function for local protein synthesis in memory storage
Synapse-specific, long-form facilitation of Aplysia sensory to motor synapses: A function for local protein synthesis in memory storage
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DOI：
10.1016/s0092-8674(00)80484-5
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发表时间：
1997-12-26
期刊：
影响因子：
64.5
通讯作者：
Kandel, ER
中科院分区：
文献类型：
--
作者：
Martin, KC;Casadio, A;Kandel, ER
在长时间的突触可塑性过程中对转录的要求提出了一个问题，即突触可塑性的细胞单位是胞体及其核还是突触。为了解决这个问题，我们培养了一个分叉的APYLIA感觉神经元，使其与两个空间上分离的运动神经元形成突触。通过将5-羟色胺灌流到一个运动神经元上的突触上，我们发现一个单一的轴突分支可以经历长期的分支特异性促进。这种分支特异性的易化依赖于CREB介导的转录，并涉及仅在处理的分支上新的突触连接的生长。分支特异性的长期易化需要突触前细胞而不是突触后细胞的局部蛋白质合成。事实上，被剥夺了细胞体的突触前感觉神经元轴突能够合成蛋白质，这种蛋白质合成通过接触5-羟色胺刺激3倍。
The requirement for transcription during long-lasting synaptic plasticity has raised the question of whether the cellular unit of synaptic plasticity is the soma and its nucleus or the synapse. To address this question, we cultured a single bifurcated Apylsia sensory neuron making synapses with two spatially separated motor neurons. By perfusing serotonin onto the synapses made onto one motor neuron, we found that a single axonal branch can undergo long-term branch-specific facilitation. This branch-specific facilitation depends on CREB-mediated transcription and involves the growth of new synaptic connections exclusively at the treated branch. Branch-specific long-term facilitation requires local protein synthesis in the presynaptic but not the postsynaptic cell. In fact, presynaptic sensory neuron axons deprived of their cell bodies are capable of protein synthesis, and this protein synthesis is stimulated 3-fold by exposure to serotonin.