In vivo estrogenic comparisons of Trifolium pratense (red clover) Humulus lupulus (hops), and the pure compounds isoxanthohumol and 8-prenylnaringenin
In vivo estrogenic comparisons of Trifolium pratense (red clover) Humulus lupulus (hops), and the pure compounds isoxanthohumol and 8-prenylnaringenin
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DOI：
10.1016/j.cbi.2008.06.005
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发表时间：
2008-10-22
影响因子：
5.1
通讯作者：
Bolton, Judy L.
中科院分区：
文献类型：
--
作者：
Overk, Cassia R.;Guo, Jian;Bolton, Judy L.
目前尚无安全可靠的激素替代疗法治疗更年期症状，因此有必要寻找替代疗法。目的：本研究的目的是利用去卵巢的成年子宫营养不良大鼠模型，评价植物膳食补充剂红三叶(红三叶草)和狼尾草(啤酒花)，以及从狼尾草中提取的两种化合物异黄腐酚(Ioxanthohumol)和8-戊烯基柚皮苷(8-Pn)的体内雌激素效应。红豆杉提取物和30%异黄酮萃取物分别以3个递增剂量和1个剂量的异黄腐酚灌胃21d，羽扇豆根中的主要雌激素8-丙基柚皮苷也以3个相应的递增剂量进行了测定。为了确定8-PN在体内的代谢，还鉴定了主要的I相和11相代谢物。主要的结局指标，子宫重量增加，表明羽扇豆杉和草鱼对子宫没有雌激素效应，次要结果指标都不是阳性的。相反，在中、高剂量8-PN有效的情况下，8-PN有明显的剂量反应。测定大鼠血浆、肝脏和乳腺中8-Prenylnaringenin的含量，并检测主要的I、II相8-PN代谢物。我们的发现表明，虽然狼尾草和草莓提取物对大鼠子宫没有影响，但与以前用于人类的相同剂量的8-PN确实有影响，因此可能对女性有有害影响。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
The lack of a safe and reliable alternative to hormone therapy (HT) for treating menopausal symptoms underscores the need for alternative therapies.Objective: The purpose of this study was to assess the in vivo estrogenic effects of the botanical dietary supplements Trifolium pratense (red clover) and Humulus lupulus (hops), and two compounds obtained from H. lupulus, isoxanthohumol and 8-prenylnaringenin (8-PN) using the ovariectomized uterotrophic adult rat model. A H. lupulus extract and a 30% isoflavone extract of T. pratense were tested at three escalating doses as was one dose of isoxanthohumol for 21 d. 8-Prenylnaringenin, the major estrogen in H. lupulus, was also tested at three relevant escalating doses. In order to determine the in vivo metabolism of 8-PN, the major phases I and 11 metabolites were also identified. The primary outcome measure, uterus weight gain, indicated that H. lupulus and T pratense did not have an estrogenic effect on the uterus, and none of the secondary outcome measures were positive. In contrast, there was a clear dose response when 8-PN was evaluated where the middle and high doses of 8-PN were active. 8-Prenylnaringenin in rat plasma, liver, and mammary gland was measured and the major phases I and II 8-PN metabolites were detected. Our findings suggest that while both the H. lupulus and T pratense extracts do not have an effect on the rat uterus, 8-PN at equivalent doses to those previously used in humans did have an effect, and may therefore have a deleterious effect in women. (c) 2008 Elsevier Ireland Ltd. All rights reserved.