Induction of a protective response with an IgA monoclonal antibody against Mycobacterium tuberculosis 16 kDa protein in a model of progressive pulmonary infection
Induction of a protective response with an IgA monoclonal antibody against Mycobacterium tuberculosis 16 kDa protein in a model of progressive pulmonary infection
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DOI：
10.1016/j.ijmm.2008.10.007
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发表时间：
2009-08-01
影响因子：
4.1
通讯作者：
Acosta, Armando
中科院分区：
文献类型：
--
作者：
Lopez, Yamile;Yero, Daniel;Acosta, Armando
结核分枝杆菌是一种兼性胞内病原体，其细胞介导的免疫被认为是免疫应答的主要组成部分。几十年来，流行的科学观点一直是抗体在改变结核分枝杆菌感染过程中几乎没有作用。近年来，一些研究已经挑战了这一教条，并且有大量证据支持抗结核分枝杆菌抗体的作用。在本工作中，我们评估了两种单克隆抗体（TBA 61和TBA 84）的保护活性。在这里，我们选择了肺部感染的肺内模型来评估细菌负荷以及治疗小鼠肺中的形态计量学和组织学变化。获得的数据显示，与用TBA 84处理的小鼠和对照小鼠相比，在用结核分枝杆菌H37Rv感染后21天用TBA 61处理的小鼠中细菌负荷减少，并且形态测量和组织病理学变化较轻。这些结果允许继续探索单克隆抗体作为针对细胞内病原体如M.结核(C)2008年Elsevier GmbH。All rights reserved.
Mycobacterium tuberculosis is a facultative intracellular pathogen for which cell-mediated immunity is considered the major component of the immune response. For many decades, the prevailing scientific view has been the antibodies have little or no role in modifying the course of M tuberculosis infection. In recent years, several studies have challenged this dogma, and there is a body of evidence that supports a role of antibodies against M tuberculosis. In the present work, we evaluated the protective activity of two monoclonal antibodies (TBA61 and TBA84). Here, we chose the intratracheal model of pulmonary infection to evaluate bacterial load and morphometric and histological changes in the lungs of treated mice. Data obtained revealed the reduction of bacterial load and milder morphometric and histopathological changes in mice treated with TBA61 at 21 days post-infection with M tuberculosis H37Rv compared to those treated with TBA84 and control mice. These results allow continuing exploring the potential use of monoclonal antibodies as prophylactic and therapeutic agents against intracellular pathogens such as M. tuberculosis. (C) 2008 Elsevier GmbH. All rights reserved.