A sensitive near-infrared fluorescent probe for caspase-mediated apoptosis: Synthesis and application in cell imaging.
A sensitive near-infrared fluorescent probe for caspase-mediated apoptosis: Synthesis and application in cell imaging.
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DOI：
10.5582/ddt.2011.v5.5.220
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发表时间：
2011-01-01
影响因子：
3.1
通讯作者：
Forrest ML
中科院分区：
文献类型：
--
作者：
Luan Y;Yang Q;Xie Y;Duan S;Cai S;Forrest ML
近红外（NIR）吸收染料代表了一个有趣的途径，从活体提取生物信息，因为它们可以监测与非侵入性光学成像技术。我们设计并合成了一种含有近红外荧光染料（IR 780）和肽基氟甲基酮（FMK）的成像剂，用于凋亡细胞的caspase-9成像。IR 780-FMK荧光探针具有79 nm的Strokes位移和0.75的量子产率。使用低至0.1 μM的IR 780-FMK成功地对经历凋亡的前列腺癌DU 145细胞进行成像。
Near-infrared (NIR) absorbing dyes represent an intriguing avenue for extracting biological information from living subjects since they can be monitored with noninvasive optical imaging techniques. We designed and synthesized an imaging agent which contains a NIR fluorochrome (IR780) and peptidyl fluoromethyl ketone (FMK) for caspase-9 imaging of cells undergoing apoptosis. The IR780-FMK fluorescent probe had a Strokes shift of 79 nm and quantum yield 0.75. Prostate cancer DU145 cells undergoing apoptosis were successfully imaged using as little as 0.1 μM of IR780-FMK.