RNA-directed DNA synthesis in Moloney murine leukemia virus: interaction between the primer tRNA and the genome RNA
RNA-directed DNA synthesis in Moloney murine leukemia virus: interaction between the primer tRNA and the genome RNA
复制标题
莫洛尼鼠白血病病毒中 RNA 指导的 DNA 合成：引物 tRNA 与基因组 RNA 之间的相互作用
作者：
G. Peters;J. Dahlberg
在Moloney小鼠白血病病毒的病毒粒子中启动RNA引导的DNA合成需要细胞内的tRNAPro作为引物。在Moloney鼠白血病病毒基因组核糖核酸上，功能引物分子结合的位置(S)和纯化的tRNAPro杂交的位置位于基因组5‘端附近(不到20%)。在tRNAPro的氨基酸受体茎和Moloney鼠白血病病毒35S RNA中的互补序列之间形成了相对稳定的双链(发生50%解离的温度，76℃)。相互作用涉及19个碱基对，从tRNAPro 3‘端的倒数第二个核苷酸延伸而来，但显然不包括3’末端的腺苷残基。在大多数方面，Moloney鼠白血病病毒中的引物和模板之间的相互作用与禽白血病-肉瘤病毒中的情况相似。
Initiation of RNA-directed DNA synthesis in virions of Moloney murine leukemia virus requires a cellular tRNAPro as primer. The site(s) on the Moloney murine leukemia virus genome RNA at which functional primer molecules are bound and at which purified tRNAPro hybridizes has been located near (within 20%) the 5' end of the genome. A relatively stable duplex (temperature at which 50% dissociation has occurred, 76 degrees C) is formed between the amino acid acceptor stem of the tRNAPro and a complementary sequence in the Moloney murine leukemia virus 35S RNA. The interaction involves 19 base pairs, extending from the penultimate nucleotide at the 3' end of the tRNAPro but apparently not including the 3'-terminal adenosine residue. In most respects, the interaction between primer and template in Moloney murine leukemia virus parallels the situation in the avian leukosis-sarcoma viruses.