Lymphocyte apoptosis, caspase activation and inflammatory response in septic shock
Lymphocyte apoptosis, caspase activation and inflammatory response in septic shock
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DOI：
10.1007/s15010-008-7070-y
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发表时间：
2008-10-01
期刊：
影响因子：
7.5
通讯作者：
Moretti, S.
中科院分区：
文献类型：
--
作者：
Delogu, G.;Famularo, G.;Moretti, S.
背景资料：我们检测了感染性休克中淋巴细胞凋亡、半胱氨酸天冬氨酸蛋白酶-1、-3、-8和-9的活性以及这两个事件与炎症反应之间的关系。在诊断为感染性休克后24小时内从16名患者中采集血液样品，以测量细胞凋亡、半胱天冬酶-3、半胱天冬酶-8、半胱天冬酶-9表达，外周血单核细胞（PBMCs）线粒体跨膜电位和Fas/FasL系统表达的变化。结果：感染性休克患者外周血单个核细胞（PBMC）凋亡率明显高于正常对照组（P < 0. 05），而感染性休克患者外周血单个核细胞（PBMC）凋亡率明显高于正常对照组（P < 0. 05（39.10 +/- 7.33% vs 4.19 +/- 1.13%; p < 0.001），半胱天冬酶-3、-8和-9的过度表达（caspase-3、-8和9分别为p < 0.01、p < 0.05、p < 0.001）以及Fas/FasL系统（p < 0.05）和线粒体跨膜电位的显著变化。脓毒症患者的胱天蛋白酶-1（101.5 +/- 18.2 pg/ml vs 9.09 +/- 2.7 pg/ml，p < 0.001）和IL-6、IL-12、IL-15和IL-18的血液水平显著高于对照组（分别为p < 0.0001、p <0.05、p < 0.05和p < 0.0001）。此外，观察到IL-6血液水平与PBMC的凋亡率（r（2）= 0.75; p = 0.001）和半胱天冬酶-9表达（r（2）= 0.92; p = 0.0001）两者相关。
Background: We examined lymphocyte apoptosis, activity of caspases-1, -3, -8 and -9 and the relationship between those two events and inflammation response in septic shock.Materials and Methods: Blood samples were obtained within 24 h after diagnosis of septic shock from 16 patients to measure apoptosis, caspases-3, -8, -9 expression, changes in mitochondrial transmembrane potential and expression of Fas/FasL system of peripheral blood monuclear cells (PBMCs). Moreover, serum levels of caspase-1 and blood concentrations of IL-6, IL-12, IL-15 and IL-18 were assessed.Results: PBMCs from patients with septic shock compared to control individuals exhibited a greatly increased frequency of apoptosis (39.10 +/- 7.33% vs 4.19 +/- 1.13%; p < 0.001), an over expression of caspases-3, -8, and -9 (p < 0.01, p < 0.05, p < 0.001 for caspases-3, -8 and -9, respectively) as well as of Fas/FasL system (p < 0.05) and significant changes in mitochondrial transmembrane potential. Blood Levels of caspase-1 (101.5 +/- 18.2 pg/ml vs 9.09 +/- 2.7 pg/ml, p < 0.001) and of IL-6, IL-12, IL-15 and IL-18 were significantly higher in septic patients vs control (p < 0.0001, p < 0.05, p < 0.05 and p < 0.0001, respectivety). Furthermore, a correlation linking IL-6 blood level with both the apoptotic rate (r(2) = 0.75; p = 0.001) and caspase-9 expression (r(2) = 0.92; p = 0.0001) of PBMCs was observed.