Glutamate synthase MoGlt1-mediated glutamate homeostasis is important for autophagy, virulence and conidiation in the rice blast fungus
Glutamate synthase MoGlt1-mediated glutamate homeostasis is important for autophagy, virulence and conidiation in the rice blast fungus
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谷氨酸合酶 MoGlt1 介导的谷氨酸稳态对于稻瘟病真菌的自噬、毒力和分生孢子形成非常重要。
DOI：
10.1111/mpp.12541
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发表时间：
2018-03-01
影响因子：
4.9
通讯作者：
Peng, You-Liang
中科院分区：
文献类型：
--
作者：
Zhou, Wei;Shi, Wei;Peng, You-Liang
谷氨酸稳态在中枢氮代谢中起着至关重要的作用，并协调几个关键的代谢功能。然而，其在真菌发病和发育中的功能尚未得到详细研究。在这项研究中，我们确定和特点的谷氨酸合酶基因MoGLT 1的稻瘟病菌是重要的谷氨酸稳态。MoGLT 1组成型表达，但在附着胞中表达量最高。MoGLT 1的缺失导致分生孢子和毒力的显着降低。ΔMoglt1突变体在附着胞穿透和侵入菌丝在穿透的植物细胞中的分化和扩散方面存在缺陷。外源谷氨酸的加入部分地挽救了ΔMoglt1突变体在产孢和植物侵染方面的缺陷。MoAtg8表达和定位的测定表明ΔMoglt1突变体在自噬中有缺陷。ΔMoglt1突变体延迟了糖原和脂体从分生孢子向发育中的附着胞的移动。总之，我们的研究结果表明，谷氨酸合酶MoGlt1介导的谷氨酸稳态是重要的发病机制和发展的稻瘟病菌，可能通过调节自噬。
Glutamate homeostasis plays a vital role in central nitrogen metabolism and coordinates several key metabolic functions. However, its function in fungal pathogenesis and development has not been investigated in detail. In this study, we identified and characterized a glutamate synthase gene MoGLT1 in the rice blast fungus Magnaporthe oryzae that was important to glutamate homeostasis. MoGLT1 was constitutively expressed, but showed the highest expression level in appressoria. Deletion of MoGLT1 resulted in a significant reduction in conidiation and virulence. The ΔMoglt1 mutants were defective in appressorial penetration and the differentiation and spread of invasive hyphae in penetrated plant cells. The addition of exogenous glutamic acid partially rescued the defects of the ΔMoglt1 mutants in conidiation and plant infection. Assays for MoAtg8 expression and localization showed that the ΔMoglt1 mutants were defective in autophagy. The ΔMoglt1 mutants were delayed in the mobilization of glycogens and lipid bodies from conidia to developing appressoria. Taken together, our results show that glutamate synthase MoGlt1-mediated glutamate homeostasis is important for pathogenesis and development in the rice blast fungus, possibly via the regulation of autophagy.