Increasing HIV-1 Molecular Complexity Among Men Who Have Sex with Men in Bangkok
Increasing HIV-1 Molecular Complexity Among Men Who Have Sex with Men in Bangkok
复制标题
DOI：
10.1089/aid.2014.0139
复制
发表时间：
2015-04-01
影响因子：
1.5
通讯作者：
Curlin, Marcel E.
中科院分区：
文献类型：
--
作者：
Leelawiwat, Wanna;Rutvisuttinunt, Wiriya;Curlin, Marcel E.
在泰国，新的HIV-1感染主要集中在某些风险群体，如男男性行为者（MSM），其年发病率可能高达每年12%。泰国男男性行为者中HIV-1分子流行病学信息的缺乏限制了对这一流行病的了解和开发新的预防方法。我们评估了2006年至2011年参加曼谷MSM队列研究（BMCS）的血清事件和血清阳性HIV-1感染男性的HIV-1亚型。我们采用多重杂交试验（MHA）对231例血清阳性和194例血清阳性受试者的HIV-1亚型进行了鉴定。通过靶向基因组测序进一步表征MHA无法分型的明显双重感染、重组菌株和分离株。大多数受试者感染了HIV-1 CRF01_AE（82%），其次是重组体（11%，主要是CRF01_AE/B重组体）、亚型B（5%）和双重感染（2%）。在CRF 01 B_AE/B重组体中观察到超过11种不同的嵌合模式，大多数涉及整合酶内的重组。2006年至2011年期间，在血清事件MSM中观察到不可分型菌株的比例显著增加。CRF01_AE和B亚型分别是最常见和最不常见的感染菌株。在参加BMCS的泰国男男性行为者中，CRF01_AE在HIV-1感染者中占主导地位，这与在泰国异性恋者和注射毒品使用者中观察到的趋势相似。复杂重组体的存在和不可分型毒株的显著增加表明，泰国HIV-1流行病的基因组成正在发生变化，这可能对HIV-1预防工作和疫苗开发构成挑战。
In Thailand, new HIV-1 infections are largely concentrated in certain risk groups such as men who have sex with men (MSM), where annual incidence may be as high as 12% per year. The paucity of information on the molecular epidemiology of HIV-1 in Thai MSM limits progress in understanding the epidemic and developing new prevention methods. We evaluated HIV-1 subtypes in seroincident and seroprevalent HIV-1-infected men enrolled in the Bangkok MSM Cohort Study (BMCS) between 2006 and 2011. We characterized HIV-1 subtype in 231 seroprevalent and 194 seroincident subjects using the multihybridization assay (MHA). Apparent dual infections, recombinant strains, and isolates found to be nontypeable by MHA were further characterized by targeted genomic sequencing. Most subjects were infected with HIV-1 CRF01_AE (82%), followed by infections with recombinants (11%, primarily CRF01_AE/B recombinants), subtype B (5%), and dual infections (2%). More than 11 distinct chimeric patterns were observed among CRF01B_AE/B recombinants, most involving recombination within integrase. A significant increase in the proportion of nontypeable strains was observed among seroincident MSM between 2006 and 2011. CRF01_AE and subtype B were the most and least common infecting strains, respectively. The predominance of CRF01_AE among HIV-1 infections in Thai MSM participating in the BMCS parallels trends observed in Thai heterosexuals and injecting drug users. The presence of complex recombinants and a significant rise in nontypeable strains suggest ongoing changes in the genetic makeup of the HIV-1 epidemic in Thailand, which may pose challenges for HIV-1 prevention efforts and vaccine development.