Three-Dimensional Circular Surface Curvature of a Spherule-Based Electrode for Selective Signaling and Dynamic Mobility of Norepinephrine in Living Cells
Three-Dimensional Circular Surface Curvature of a Spherule-Based Electrode for Selective Signaling and Dynamic Mobility of Norepinephrine in Living Cells
复制标题
基于小球的电极的三维圆形表面曲率，用于活细胞中去甲肾上腺素的选择性信号传导和动态迁移
DOI：
10.1021/acsabm.0c00882
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发表时间：
2020
影响因子：
4.7
通讯作者：
Elmarakbi Ahmed
中科院分区：
文献类型：
--
作者：
Emran Mohammed Y.;Shenashen Mohamed A.;El-Safty Sherif A.;Selim Mahmoud M.;Minowa Takashi;Elmarakbi Ahmed
在一些神经疾病的临床研究中，应建立一种高灵敏度的人体体液和神经细胞系模型中去甲肾上腺素（NEP）信号的检测方案。设计了一种基于硫掺杂碳球表面（S-CSN）的无金属电极催化剂，并将其作为生物样品中NEP选择性信号转导元件。所设计的S-CSN电极具有球形结构和曲率表面，形成平均层尺寸<2 nm的球形纳米层。S-CSN的表面形貌为圆形曲面曲率，具有凹凸不平的表面纹理、脊端和夹层之间的自由开放空间。具有丰富空间分异的S-CSN表面具有分子/电子扩散速度快、扩散中心多、靶载荷高、面内外圆等特点。S原子在碳基电极上的活性掺杂产生了具有许多活性位点的主动转导元件，与目标分子的强结合，电荷/分子的易于扩散，长期耐用性和密集的反应暴露位点，用于在超痕量水平上发出NEP信号。S-CSN可以作为一种灵敏、选择性的纳米传感器，用于NEP信号的发送，并建立具有高稳定性和可重复性的传感协议。基于S-CSN的传感协议具有较高的灵敏度和选择性，检测限低至0.001 μM，线性范围宽至0.01 ~ 0.8 μM。活细胞（神经细胞系模型）在刺激剂作用下分泌NEP的体外感觉方案具有高灵敏度、低细胞毒性和高生物相容性。这些结果证实了在人类血液样本和神经细胞中成功建立NEP传感器用于临床研究。
A highly sensitive protocol for signaling norepinephrine (NEP) in human fluids and neuronal cell line models should be established for clinical investigation of some neuronal diseases. A metal-free electrode catalyst was designed based on a sulfur-doped carbon spheroidal surface (S-CSN) and employed as a transducing element for selective signaling of NEP in biological samples. The designed electrode of S-CSN features a spherical construct and curvature surface to form a spheroidal nanolayer with an average layer size of <2 nm. S-CSN shows surface topography of a circular surface curvature with a rugged surface texture, ridge ends, and free open spaces between interlayers. The rich-space diversity surfaces offer highly active surface with facile molecular/electron diffusion, multi-diffusive centers, and high target loading along with in-/out-of-plane circular spheres of the S-CSN surface. The active doping of S atoms onto the carbon-based electrode creates an active transducing element with many active sites, strong binding to targeted molecules, facile diffusion of charges/molecules, long-term durability, and dense reactive exposure sites for signaling NEP at ultratrace levels. S-CSN could be a sensitive and selective nanosensor for signaling NEP and establishing a sensing protocol with high stability and reproducibility. The sensory protocol based on S-CSN exhibits high sensitivity and selectivity with a low detection limit of 0.001 μM and a wide linear range of 0.01–0.8 μM. The in vitro sensory protocol for NEP secreted from living cells (neuronal cell line model) under stimulated agents possesses high sensitivity, low cytotoxicity, and high biocompatibility. These results confirm the successful establishment of NEP sensor in human blood samples and neuronal cells for clinical investigation.