Protease-dependent activation of nasal polyp epithelial cells by airborne fungi leads to migration of eosinophils and neutrophils
Protease-dependent activation of nasal polyp epithelial cells by airborne fungi leads to migration of eosinophils and neutrophils
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DOI：
10.1080/00016480500395179
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发表时间：
2006-12-01
影响因子：
1.4
通讯作者：
Jeon, Chang-Ho
中科院分区：
文献类型：
--
作者：
Shin, Seung-Heon;Lee, Young-Ho;Jeon, Chang-Ho
结论.真菌中的蛋白酶与鼻上皮细胞相互作用并增强体外炎性细胞因子的产生。这些细胞因子诱导嗜酸性粒细胞和中性粒细胞的迁移。蛋白酶激活受体（PARs）也可能在上皮细胞活化过程中发挥作用。Objective.鼻上皮是空气中变应原遇到的第一道屏障，也是气道炎症的积极参与者。真菌是鼻窦炎的重要致病菌，由多种过敏原蛋白组成。材料和方法。从患者中获得鼻息肉上皮细胞，并用链格孢属、曲霉属和枝孢属刺激。测定白细胞介素-8（IL-8）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和正常T细胞表达和分泌的活化调节因子（RANTES），以确定上皮细胞的活化。采用逆转录聚合酶链反应（RTPCR）检测鼻黏膜上皮细胞PAR mRNA的表达。用鼻息肉上皮细胞条件培养基（HPECM）诱导嗜酸性粒细胞和中性粒细胞迁移。结果真菌增强鼻上皮细胞化学介质的产生。当鼻上皮细胞被真菌激活时，PAR 2和PAR 3 mRNA的表达比未激活的细胞更强。在HPECM中，RANTES和嗜酸性粒细胞趋化因子诱导嗜酸性粒细胞迁移，IL-8诱导中性粒细胞迁移。
Conclusions. Proteases in fungi interact with nasal epithelial cells and enhance the production of inflammatory cytokines in vitro. These cytokines induced the migration of eosinophils and neutrophils. Protease-activated receptors (PARs) might also play a role in the process of epithelial cell activation. Objective. The nasal epithelium is the first barrier encountered by airborne allergens and an active participant in airway inflammation. Fungi have been increasingly recognized as important pathogens in sinusitis and consist of several allergenic proteins. Materials and methods. Nasal polyp epithelial cells were obtained from patients and stimulated with Alternaria, Aspergillus, and Cladosporium. Interleukin-8 (IL-8), granulocyte-macrophage colony stimulating factor (GM-CSF), and regulated on activation normal T expressed and secreted (RANTES) were measured to determine the activation of epithelial cells. Reverse transcriptase-polymerase chain reaction test (RTPCR) for PAR mRNA expression in nasal epithelial cells was performed. Eosinophil and neutrophil migration was induced with nasal polyp epithelial cells conditioned media (HPECM). Results. Fungi enhanced the production of chemical mediators from nasal epithelial cells. When nasal epithelial cells were activated with fungi, PAR2 and PAR3 mRNAs were more strongly expressed than in nonactivated cells. Eosinophil migration was induced by RANTES and eotaxin, and neutrophil migration was induced by IL-8 in HPECM.