DIFFERENTIAL LOCALIZATION OF 2 MYOSINS WITHIN NEMATODE THICK FILAMENTS
DIFFERENTIAL LOCALIZATION OF 2 MYOSINS WITHIN NEMATODE THICK FILAMENTS
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DOI：
10.1016/0092-8674(83)90381-1
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
EPSTEIN, HF
中科院分区：
文献类型：
--
作者：
MILLER, DM;ORTIZ, I;EPSTEIN, HF
线虫的体壁肌肉细胞含有两种独特的肌球蛋白H链，A和B。用免疫化学方法确定了这两种肌球蛋白在体壁肌肉粗纤维中的结构位置。在免疫荧光显微镜下，肌球蛋白B抗体标记除A带中心的细小间隙外，体壁肌肉中含有粗丝的A带；肌球蛋白A抗体在每个A带内反应形成一条中间的荧光条。将这些单抗与分离的粗丝形成的复合体进行了负染色，并进行了电镜观察。肌球蛋白B抗体与所有细丝的极区反应，但不与中央0.9µm区反应。肌球蛋白A抗体与所有细丝中的中心1.8µm区域反应，但不与极区反应。
The body wall muscle cells of the nematode, C. elegans, contain 2 unique types of myosin H chain, A and B. An immunochemical approach was used to define the structural location of these 2 myosins within body wall muscle thick filaments. By immunofluorescence microscopy, myosin B antibodies label the thick filament-containing A-bands of body wall muscle with the exception of a thin gap at the center of each A-band; myosin A antibodies react to form a medial fluorescent stripe within each A-band. The complexes of these monoclonal antibodies with isolated thick filaments were negatively stained and studied by EM. The myosin B antibody reacts with the polar regions of all filaments but does not react with a central 0.9 .mu.m zone. The myosin A antibody reacts with a central 1.8 .mu.m zone in all filaments but does not react with the polar regions.