Optimization of 14C liquid scintillation counting of plant and soil lipids to trace short term formation, translocation and degradation of lipids
Optimization of 14C liquid scintillation counting of plant and soil lipids to trace short term formation, translocation and degradation of lipids
复制标题
优化植物和土壤脂质的 14C 液体闪烁计数，以追踪脂质的短期形成、易位和降解
DOI：
10.1007/s10967-010-0450-7
复制
发表时间：
2010
影响因子：
1.6
通讯作者：
Y. Kuzyakov
中科院分区：
文献类型：
--
作者：
G. Wiesenberg;M. Gocke;Y. Kuzyakov
两种强有力的方法经常被用来跟踪的掺入和降解的植物来源的C在土壤中：14 C标记/追逐和分析的脂质组成。在这项研究中，我们耦合这些方法，以跟踪短期掺入植物来源的脂质根际和非根际土壤。方法优化是必需的，并暗示14 C液体闪烁计数改善植物脂质提取物考虑到有机溶剂，溶剂闪烁鸡尾酒的比例，和量的脂质。方法优化后，14 C脂肪酸数据表明，根源脂质对根际和非根际土壤的贡献显着。耦合14 C标记/追逐与脂质分析是一个强大的和廉价的方法来跟踪根来源的C在土壤中允许估计C预算，用于确定植物内和植物到土壤的C的形成和转运，以及用于识别土壤中的特定化合物类的短期动态。
Two powerful approaches are frequently used to trace incorporation and degradation of plant derived C in soil: 14C labelling/chasing and analysis of lipid composition. In this study, we coupled these approaches in order to trace short term incorporation of plant derived lipids into rhizosphere and non-rhizosphere soil. Methodological optimization was required and implied 14C liquid scintillation counting improvement for plant lipid extracts taking into account organic solvents, solvent-to-scintillation cocktail ratio, and amount of lipids. Following method optimization, 14C data of fatty acids indicated a notable contribution of root derived lipids to rhizosphere and non-rhizosphere soil. Coupling of 14C labelling/chasing with lipid analysis is a powerful and cheap approach for tracing of root derived C in soil allowing for estimation of C budget, for determination of C formation and translocation within plants and from plant to soil, as well as for identification of short term dynamics of specific compound classes within soil.