Kaiso is expressed in lung cancer: Its expression and localization is affected by p120ctn (Retracted article. See vol. 102, pg. 141, 2010)
Kaiso is expressed in lung cancer: Its expression and localization is affected by p120ctn (Retracted article. See vol. 102, pg. 141, 2010)
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DOI：
10.1016/j.lungcan.2009.06.013
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发表时间：
2010-02-01
期刊：
影响因子：
5.3
通讯作者：
Wang, En-Hua
中科院分区：
文献类型：
--
作者：
Dai, Shun-Dong;Wang, Yan;Wang, En-Hua
背景：Kaiso是最近发现的一种与p120-catenin（p120 ctn）结合的转录因子，p120-catenin是一种犰狳连环蛋白和细胞粘附辅因子。方法：应用免疫组化方法检测196例肺癌组织（包括55例配对淋巴结转移和80例有完整随访记录）中Kaiso和p120 ctn的表达和定位。使用三种肺癌细胞系BE 1、SPC和A549建立p120 ctn稳定消融或过表达的细胞系。免疫共沉淀法检测肺癌组织和细胞系中p120 ctn与Kaiso的结合情况。结果：115例（58.7%）患者Kaiso在胞浆中表达，168例（85.7%）患者p120 ctn表达异常。胞浆Kaiso和p120 ctn异常表达与高恶性度相关（高分期和淋巴结转移，均P
Background: Kaiso is a recently identified transcription factor that binds to p120-catenin (p120ctn), an Armadillo catenin and cell adhesion cofactor. However, clinical studies of human solid tumors have not been reported to investigate relationship between these proteins.Methods: Expression and localization of Kaiso and p120ctn were examined in 196 lung cancer specimens (including 55 cases of paired lymph node metastases and 80 cases with complete follow-up records) by immunohistochemistry. Three lung cancer cell lines, BE1, SPC, and A549 were used to establish p120ctn stably ablated or overexpressed cell lines. Co-immunoprecipitation was used to confirm p120ctn bind Kaiso in lung cancer tissue and cell lines. Localization and expression levels of Kaiso were detected via immunofluorescence, cytoplasmic vs. nuclear fractionation Western blot analysis and reverse transcription-polymerase chain reaction.Results: Cytoplasmic Kaiso expression was evident in 115 (58.7%), and abnormal p120ctn expression was noted in 168 (85.7%). Cytoplasmic Kaiso and abnormal p120ctn expressions were associated with higher degree of malignancy (high-stage and lymph node metastases, all P