Naturally-acquired humoral immune responses against the N- and C-termini of the Plasmodium vivax MSP1 protein in endemic regions of Brazil and Papua New Guinea using a multiplex assay.
Naturally-acquired humoral immune responses against the N- and C-termini of the Plasmodium vivax MSP1 protein in endemic regions of Brazil and Papua New Guinea using a multiplex assay.
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DOI：
10.1186/1475-2875-9-29
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发表时间：
2010-01-21
期刊：
影响因子：
3
通讯作者：
del Portillo HA
中科院分区：
文献类型：
--
作者：
Fernandez-Becerra C;Sanz S;Brucet M;Stanisic DI;Alves FP;Camargo EP;Alonso PL;Mueller I;del Portillo HA
间日疟原虫是分布最广的人类疟疾寄生虫，要在研制疟疾疫苗方面取得进展，就必须更好地了解在疟疾流行地区为病人提供临床保护的免疫反应。将代表间日疟原虫裂殖子表面蛋白1的N-末端PvMSP 1-N和C-末端PvMSP 1-C的谷胱甘肽S-转移酶（GST）和GST-融合蛋白共价偶联至BioGlycoprotein羧化珠粒。在ELISA和Bioplex测定中分别使用重组蛋白和偶联的珠粒，所述测定使用来自巴西和巴布亚新几内亚的间日疟原虫患者的免疫血清来测定IgG和亚类应答。使用Kappa统计量和斯皮尔曼秩相关性评价两种方法在血清阳性应答中的一致性。使用这种方法的结果进行了比较与经典的微量酶联免疫吸附试验（ELISA），表明该测定是敏感的，可重复的，并具有良好的一致性与ELISA，然而，进一步研究不同的统计分析似乎是可取的，然后才声称结论性的结果完全基于多重测定。正如预期的那样，结果表明，PvMSP 1在来自巴西和巴布亚新几内亚（PNG）不同流行地区的患者的自然感染中具有免疫原性，并且年龄仅与针对该分子C末端部分的抗体相关。此外，这些流行地区的IgG亚类谱是不同的，巴西的IgG 3主要识别PvMSP 1，巴布亚新几内亚的IgG 1主要识别PvMSP 1。本研究验证了使用多重测定来测量天然获得的抗间日疟原虫裂殖子表面蛋白1的IgG抗体。
Progress towards the development of a malaria vaccine against Plasmodium vivax, the most widely distributed human malaria parasite, will require a better understanding of the immune responses that confer clinical protection to patients in regions where malaria is endemic. Glutathione S-transferase (GST) and GST-fusion proteins representing the N- terminus of the merozoite surface protein 1 of P. vivax, PvMSP1-N, and the C-terminus, PvMSP1-C, were covalently coupled to BioPlex carboxylated beads. Recombinant proteins and coupled beads were used, respectively, in ELISA and Bioplex assays using immune sera of P. vivax patients from Brazil and PNG to determine IgG and subclass responses. Concordances between the two methods in the seropositivity responses were evaluated using the Kappa statistic and the Spearman's rank correlation. The results using this methodology were compared with the classical microtitre enzyme-linked immnosorbent assay (ELISA), showing that the assay was sensitive, reproducible and had good concordance with ELISA; yet, further research into different statistical analyses seems desirable before claiming conclusive results exclusively based on multiplex assays. As expected, results demonstrated that PvMSP1 was immunogenic in natural infections of patients from different endemic regions of Brazil and Papua New Guinea (PNG), and that age correlated only with antibodies against the C-terminus part of the molecule. Furthermore, the IgG subclass profiles were different in these endemic regions having IgG3 predominantly recognizing PvMSP1 in Brazil and IgG1 predominantly recognizing PvMSP1 in PNG. This study validates the use of the multiplex assay to measure naturally-acquired IgG antibodies against the merozoite surface protein 1 of P. vivax.
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DOI：
10.4269/ajtmh.2005.73.244
发表时间：
2005-08-01
影响因子：
3.3
作者：
Tran, TM;Oliveira-Ferreira, J;Galinski, MR
通讯作者：
Galinski, MR
影响因子：
4
作者：
Wickramarachchi, Thilan;Illeperuma, Ruwan J.;Udagama-Randeniya, Preethi V.
通讯作者：
Udagama-Randeniya, Preethi V.
DOI：
10.4269/ajtmh.2002.66.641
发表时间：
2002-06-01
影响因子：
3.3
作者：
Alves, FP;Durlacher, RR;Camargo, EP
通讯作者：
Camargo, EP
中国按蚊群（双翅目：蚊科）：核糖体 DNA 内转录间隔区 2 序列推断的物种区分和系统发育关系
影响因子：
2.1
作者：
Afrane, YA;Lawson, BW;Yan, GY
通讯作者：
Yan, GY
DOI：
10.4269/ajtmh.2007.76.997
发表时间：
2007-06-01
影响因子：
3.3
作者：
Michon, Pascal;Cole-Tobian, Jennifer L.;Mueller, Ivo
通讯作者：
Mueller, Ivo