Visualization of Sulfane Sulfur in Plants with a Near-Infrared Fluorescent Probe
Visualization of Sulfane Sulfur in Plants with a Near-Infrared Fluorescent Probe
复制标题
使用近红外荧光探针可视化植物中的硫烷硫
DOI：
10.1021/acssensors.8b01423
复制
发表时间：
2019-02-01
期刊：
影响因子：
8.9
通讯作者：
Liu, Changlin
中科院分区：
文献类型：
--
作者：
Jiang, Gangwei;Li, Man;Liu, Changlin
磺胺类硫是一类重要的活性硫。这些化合物具有独特的反应活性，可与其他硫原子可逆地结合，并在不同的生物系统中表现出调节作用。近年来的研究表明，亚硫酸盐参与了硫化氢（H2S）的信号转导过程。开发具有选择性、快速、灵敏检测磺胺硫的探针，对于研究其在生物系统中的生理病理作用，特别是在生理研究相对滞后的植物系统中具有重要意义。然而，到目前为止，仍然缺乏足够的化学工具来直接跟踪和测量生物系统中的磺胺硫，特别是对植物活体组织中的磺胺硫的检测仍然具有挑战性。在此，我们报道了一种近红外荧光探针，SSNIP，用于选择性成像的亚硫酸盐硫。SSNIP能够在水缓冲液和活的人细胞中检测生理浓度的磺胺硫。然后，我们利用SSNIP首次展示了植物组织（如拟南芥根系）内源磺胺硫的荧光监测。应用SSNIP技术评价拟南芥不同生育期根系中硫的含量。结果表明，拟南芥根系中硫的含量水平与其生长阶段密切相关，表明硫可能是促进植物生长和根系伸长的信号分子。此外，该研究还揭示了其在植物生理学、生物学和病理学研究方面的潜在应用前景。
Sulfane sulfur species are an important type of reactive sulfur species. These compounds have unique reactivity to attach reversibly to other sulfur atoms and exhibit regulatory effects in diverse biological systems. Recent studies have suggested that sulfane sulfurs are involved in signal transduction processes of hydrogen sulfide (H2S). The development of probes for selective, rapid, and sensitive detection of sulfane sulfur is of great significance for studying their physiological and pathological roles in biological systems, especially in plant systems for which physiological research has lagged behind. However, so far there is still a lack of sufficient chemical tools for directly tracking and measuring sulfane sulfur in biological systems, and in particular, the detection of sulfane sulfur in living plant tissues is still challenging. Herein, we report a near-infrared fluorescent probe, SSNIP, for the selective imaging of sulfane sulfur. SSNIP is capable of detecting sulfane sulfur at physiological concentrations in both aqueous buffer and living human cells. Then, with SSNIP, we demonstrate the fluorescent monitoring of endogenous sulfane sulfur in plant tissues such as Arabidopsis thaliana roots for the first time. Furthermore, the application of SSNIP in evaluating the level of sulfane sulfur in Arabidopsis thaliana roots at different growth stages is performed. The results show that the level of sulfane sulfur in Arabidopsis thaliana roots correlates well with their growth stages, which suggests that sulfane sulfurs might act as actual signaling molecules to promote plant growth and root elongation. In addition, it reveals potential applications for the biological and pathological studies of sulfane sulfur, especially in plant physiology.