Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish
Acquisition, Processing and Analysis of Electrocardiogram in Awake Zebrafish
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DOI：
10.1109/jsen.2019.2897789
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发表时间：
2019-06-01
影响因子：
4.3
通讯作者：
Cao, Hung
中科院分区：
文献类型：
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作者：
Le, Tai;Lenning, Michael;Cao, Hung
长期监测斑马鱼的内源性心电图（ECG）在心脏病研究和药物筛选中起着至关重要的作用。在本文中，我们开发了一种基于聚合物的嵌入式柔性薄膜电极的装置来获取清醒斑马鱼的心电信号。该装置由聚二甲基硅氧烷使用模塑技术制成，其中模具通过3D打印形成。在National Instruments LabView平台上构建了图形用户界面，用于实时记录、处理和分析。该程序提供了信号去噪、特征波检测、异常检测等重要功能。此外，它可以对实时到来的信号和先前保存的数据进行操作，从而促进分析和解释。我们展示了使用我们的系统来研究麻醉药物，即Tricaine（MS-222），对斑马鱼心脏电生理的影响，揭示了有希望的发现。我们推测，我们的新系统可能有助于使用斑马鱼模型在各个学科中进行大量研究。
Long-term monitoring of intrinsic electrocardiogram (ECG) in zebrafish plays a crucial role in heart disease studies and drug screening. In this paper, we developed a polymer-based apparatus with embedded flexible thin-film electrodes to acquire ECG signals of awake zebrafish. The apparatus was made of polydimethylsiloxane using the molding technique with molds formed by 3D printing. A graphical user interface was built in National Instruments LabView platform for real-time recording, processing, and analysis. The program provided important features, such as signal de-noising, characteristic wave detection, and anomaly detection. Further, it could operate on both real-time coming signals and previously-saved data, facilitating analysis and interpretation. We demonstrated the use of our system to investigate the effects of the anesthetic drug, namely Tricaine (MS-222), on cardiac electrophysiology of zebrafish, revealing promising findings. We speculate that our novel system may contribute to a host of studies in various disciplines using the zebrafish model.